Novel features of the XRN-family in Arabidopsis: Evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm -- Data Supplement - Supplemental Figure -- Proceedings of the National Academy of Sciences

Supplementary material for Kastenmayer and Green (2000) Proc. Natl. Acad. Sci. USA 97 (25), 13985-13990.

Supplemental Figure 5 Fig. 5.
AtXRN2-GFP and AtXRN4-GFP localization. (A) AtXRN4-GFP was expressed in the rat1-1^ts strain, and GFP fluorescence was compared with DAPI-stained nuclear DNA. The GFP and DAPI images of the boxed cells are shown superimposed (overlay). (B) Plasmids encoding AtXRN2-GFP and AtXRN4-GFP were used to transform onion epidermal cells by particle bombardment. GFP fluorescence of AtXRN2-GFP and AtXRN4-GFP was compared to that of GFP-GUS, a primarily cytoplasmic protein, and GFP-NIa, which is targeted to the nucleus. The onion epidermal cell images were obtained with a ×20 objective. A ×40 image of AtXRN4-GFP 1 µg is also shown. The arrows indicate the nuclei.