Supplementary material for Anderson et al. (1999) Proc. Natl. Acad. Sci. USA 96(16), 9311-9316.
Table 2. T cell receptor a (TCRa)-specific cDNA libraries were prepared by anchor-PCR using total cellular RNA extracted from islet-derived T cells of five diabetic 8.3-TCRb-transgenic mice, from nonobese diabetic (NOD)-islet stimulated (3 stimulations a 1-week intervals) or NRP-stimulated (2 stimulations at 1-week intervals) CD8+ T cells of three nondiabetic 8.3-TCRb-NOD mice, and from pooled CD4+ T cell-depleted splenocytes from three nondiabetic 8.3-TCRb-NOD mice. The TCRa cDNAs of each line were cloned and sequenced. The table shows the total number of copies of each sequence/total number of cDNAs sequenced (N). N-terminal residues homologous to those of the 8.3-TCRa chain (top sequence) are underlined.