Activity-based probes for proteomic profiling of histone deacetylase complexes -- Salisbury and Cravatt 104 (4): 1171 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Salisbury and Cravatt 1010.1073/pnas.0608659104

Supporting Information

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SI Figure 6
SI Figure 7
SI Table 2
SI Table 3
SI Table 4
SI Text

SI Figure 6

Fig. 6. ABPP of breast, ovarian, and melanoma cancer cell proteomes with the SAHA-BPyne probe. Soluble proteomes were incubated with 100 nM SAHA-BPyne probe in the presence or absence of excess SAHA (10 mM) as a competitor. Probe targets were detected by UV-irradiation, followed by click chemistry with a rhodamine-azide tag, SDS/PAGE analysis, and in-gel fluorescence scanning. 231, MDA-MB-231 cells.

SI Figure 7

Fig. 7. ABPP of subcellular fractions of SKOV3 cancer cells with the SAHA-BPyne probe. Soluble and nuclear SKOV3 proteomes were prepared as described in the Supporting Methods and incubated with 100 nM SAHA-BPyne probe in the presence or absence of excess SAHA (S, 10 mM) or trichostatin A (T, 10 mM) as a competitor. Probe targets were detected by UV-irradiation, followed by click chemistry with a rhodamine-azide tag, SDS/PAGE analysis, and in-gel fluorescence scanning.