Gerlach et al. 10.1073/pnas.0606777104.

Supporting Information

Files in this Data Supplement:

SI Table 3
SI Table 4
SI Table 5
SI Table 6
SI Table 7
SI Figure 3
SI Table 8




Fig. 3. Choice flume (20 ´ 4 ´ 2.5 cm). Odor stimuli were presented on sides A and B. 1 = inflow, 2 = collimator to homogenize turbulences, 3 = barrier-separated channels to further laminate flow (6.7 cm long), 4 = upstream fine mesh (0.5 mm) net to contain test fish, and 5 = outflow.





Table 3. Allelic variability within reef populations of A. polyacanthus in 2005

Locus

 

OTI

S

H

L

F

 

n

41

51

45

42

57

AC 33

n

a

6

9

5

7

16.0

AY818661

%mcA

64.6

35.3

48.9

55.0

29.5

 

H

obs

0.54

0.69

0.64

0.53

0.79

 

H

exp

0.54

0.76

0.68

0.65

0.83

 

F

IS

0.009

0.094

0.053

0.175

0.056

AC 37

n

a

8

8

4

9

6

AY818662

%mcA

48.8

37.0

64.3

57.1

42.9

 

H

obs

0.56

0.72

0.38

0.60

0.57

 

H

exp

0.66

0.74

0.49

0.64

0.66

 

F

IS

0.151

0.022

0.222

0.066

0.131

AC 45

n

a

37

37

24

31

34

AY818664

%mcA

7.6

7.4

12.8

12.5

8.8

 

H

obs

0.85

0.75

0.79

0.80

0.84

 

H

exp

0.98

0.97

0.94

0.96

0.97

 

F

IS

0.135*

0.233*

0.160*

0.168*

0.128*

Pom 3

n

a

10

8

2

6

8

AY721657

%mcA

23.2

45.1

45.5

43.8

28.9

 

H

obs

0.78

0.75

0.55

0.65

0.83

 

H

exp

0.85

0.74

0.51

0.72

0.83

 

F

IS

0.087

-0.009

-0.089

0.091

0.003

Pom 15

n

a

16

19

10

14

21

AY721659

%mcA

21.4

14.1

28.8

17.7

20.7

 

H

obs

0.75

0.80

0.69

0.74

0.76

 

H

exp

0.89

0.92

0.87

0.89

0.92

 

F

IS

0.162

0.135

0.204

0.169

0.175*

All loci

F

IS

0.0116*

0.104*

0.123*

0.138*

0.100*

Locus: microsatellite marker with accession number (GenBank); n = number of individuals; na = number of alleles, %mcA = Frequency of most common allele; observed (Hobs) and expected (Hexp) heterozygosity; FIS values after correction for null alleles; * indicates statistical significance based on 500 randomizations with an indicative adjusted nominal level (5%) for multiple comparisons = 0.002.





Table 4. Allelic variability within reef populations of O. doederleini in 2005

Locus

 

OTI

S

H

L

F

 

n

77

62

39

77

59

Ad 65.2

n

a

12

11

13

10

7

AY496689

%mcA

32.7

42.9

40.9

32.8

56.5

 

H

obs

0.79

0.71

0.7

0.75

0.63

 

H

exp

0.82

0.75

0.79

0.8

0.60

 

F

IS

0.043

0.055

0.114

0.066

-0.053

Ad 67

n

a

31

31

29

26

26

AY496690

%mcA

12.9

6.7

8.8

12.3

11.5

 

H

obs

0.97

0.87

0.91

0.90

0.98

 

H

exp

0.95

0.97

0.96

0.95

0.95

 

F

IS

-0.021

0.105*

0.055

0.053

-0.032

Ad 70

n

a

34

29

29

28

31

AY496691

%mcA

12.0

12.3

10.6

8.2

12

 

H

obs

0.91

0.90

0.94

0.90

0.90

 

H

exp

0.96

0.95

0.96

0.96

0.96

 

F

IS

0.056

0.061

0.021

0.063

0.074

Ad 86.2

n

a

26

24

21

23

37

AY496693

%mcA

16.7

21.6

14.7

15.2

16.0

 

H

obs

0.85

0.76

0.72

0.75

0.83

 

H

exp

0.92

0.92

0.91

0.93

0.93

 

F

IS

0.088

0.168*

0.221*

0.192*

0.113

Ad 94

n

a

8

9

16

12

11

AY496695

%mcA

30.7

29.0

22.7

33.6

32.7

 

H

obs

0.75

0.80

0.79

0.74

0.75

 

H

exp

0.76

0.81

0.89

0.78

0.79

 

F

IS

0.005

0.017

0.116

0.046

0.062

       

All loci

F

IS

0.034

0.084*

0.104*

0.086*

0.039

Locus: microsatellite marker with accession number (GenBank); n= number of individuals; na = number of alleles, %mcA = Frequency of most common allele; observed (Hobs) and expected (Hexp) heterozygosity; FIS values after correction for null alleles; * indicates statistical significance based on 500 randomizations with an indicative adjusted nominal level (5%) for multiple comparisons = 0.002.





Table 5. Allelic variability within reef populations of P. coelestis in 2003

Locus

 

OTI

S

H

L

F

 

n

39

29

40

46

43

Pom 3

n

a

12

12

15

17

20

AY721657

%mcA

32.9

25.9

16.7

25

17.8

 

H

obs

0.89

0.79

0.79

0.78

0.81

 

H

exp

0.83

0.79

0.9

0.87

0.91

 

F

IS

-0.079

0.088

0.120

0.104

0.114

Pom 6

n

a

24

19

20

25

26

AY721658

%mcA

14.1

11.1

13.75

13.3

11.5

 

H

obs

0.82

0.96

0.85

0.84

0.79

 

H

exp

0.93

0.95

0.92

0.95

0.94

 

F

IS

0.122

0.014

0.082

0.110

161

Pom 15

n

a

36

26

32

7

34

AY721659

%mcA

8.1

10.7

11.2

8.9

11.2

 

H

obs

0.84

0.64

0.77

0.71

0.80

 

H

exp

0.98

0.97

0.96

0.97

0.96

 

F

IS

0.143*

0.338*

0.193*

0.269*

0.171

Pom 16.2

n

a

25

19

24

25

23

AY721660

%mcA

10.5

12.1

16.3

9.8

9.5

 

H

obs

0.73

0.65

0.75

0.76

0.76

 

H

exp

0.95

0.94

0.93

0.94

0.95

 

F

IS

0.229*

0.309*

0.203*

0.200*

0.200*

Pom 26

n

a

20

15

14

19

15

AY721663

%mcA

26.3

20.7

28.2

19.6

30.2

 

H

obs

0.76

0.79

0.89

0.78

0.93

 

H

exp

0.90

0.90

0.87

0.92

0.86

 

F

IS

0.155

0.123

-0.029

0.153

-0.075

All loci

F

IS

0.118*

0.116*

0.169*

0.171*

0.119*

Locus: microsatellite marker with accession number (GenBank); n = number of individuals; na = number of alleles, %mcA = Frequency of most common allele; observed (Hobs) and expected (Hexp) heterozygosity; FIS values after correction for null alleles; * indicates statistical significance based on 500 randomizations with an indicative adjusted nominal level (5%) for multiple comparisons = 0.002.





Table 6. Genetic population structure of O. doederleini 2003 at four reefs

Reefs

OTI

S

H

S

0.0191*

  

H

0.0047

0.0275*

 

L

0.0278*

0.0431*

0.0309*

Below diagonal: pairwise FST values (* indicates statistical significance after Bonferroni correction for multiple comparisons; P = 0.0083).





Table 7. Hierarchical population genetic analyses

Species

 

% variation

P

A. polyacanthus

Within reef populations

93.97

 
 

Among reef populations within groups

3.60

0.000

 

Among groups

2.43

0.22

O. doederleini

Within reef populations

98.1

 
 

Among reef populations within groups

1.87

0.000

 

Among groups

0.03

0.52

P. coelestis

Within reef populations

99.8

 
 

Among reef populations within groups

0.002

0.59

 

Among groups

0

0.35

Percentage variation (AMOVA) within and between groups (group 1: northern reefs H, OTI, and S; group 2: southern reefs L and F). P = statistical significance (α = 0.05).





Table 8. Olfactory preference of apogonids and pomacentrids for water from "home" versus all other reefs

Species

Reef comparison

Preference, %

n

P

Apogonid

 sp.

Home/L

16.5 ± 5.4

42

0.005

 

Home/FO

13.2 ± 4.5

40

0.015

 

Home/S

4.9 ± 3.1

51

0.024

 

Home/H

9.7 ± 2.1

190

0.000

Pomacentrus

 sp.

Home/S

13.2 ± 5.2

23

0.016

 

Home/H

12.9 ± 3.9

77

0.001

Preference expressed as mean difference ± SEM in time spent in home (OTI or F) versus other reef water during odor choice test in two-channel flume. Positive values indicate preference for home reef odor, and negative values indicate preference for other reef odor. Animals were caught at two different "home" reefs (OTI and F) and tested against water from four other reefs. (Wilcoxon signed-rank test: n = number of fish tested; P = statistical significance value.)