10.1073/pnas.0610491104 |
Table 1. Plasmids and strains
Plasmids/strains | Relevant genotype or description | Source |
Plasmids | ||
pMLB1113 | Low copy number vector | 1 |
pHM542 | His(5)::rhlB in pET15-b | M. Cashel, National Institutes of Health, Bethesda, MD |
pRNE1a | Plac-rne::yfp | This study |
pRNE2a | Plac-yfp::rne | This study |
pRNE5a | Plac-rne1-377::yfp | This study |
pRNE7a | Plac-rne1-417::yfp | This study |
pRNE9a | Plac-rne1-659::yfp | This study |
pRNE13a | Plac-yfp::rne378-659 | This study |
pRNE19a | Plac-yfp::rne660-1061 | This study |
pRNE26a | Plac-yfp::rne418-602 | This study |
pRNE31a | Plac-rne1-417::HA | This study |
pSU314 | HA-epitope-cat | 2 |
pSU315 | HA-epitope-kan | 2 |
pKD46 | Red recombinase expression plasmid | 3 |
pCP20 | FLP expression plasmid | 4 |
pMDB1 | Gal4BD::minD | 5 |
Strains | ||
AH109 | Host for yeast two-hybrid essay | Clontech |
MC1000 | Wt strain | 1 |
CF5961 | BL21(DE3)/pHM542 | M. Cashel |
PB103 | Wt strain | 1 |
PB114 | PB103 ∆minB | 1 |
RC1 | MC1000 ∆minB | 6 |
GC2985 | SfiA::tn5 | Richard D'Ari |
KW153 | N3433 ∆rne/pKW135 | 7 |
DY330 | Host strain for red recombination | 8 |
AT1b | MC1000 ∆rne | This study |
AT8 | MC1000 rne∆418-1061-cat | This study |
AT9 | AT8 ∆minB | This study |
AT14 | MC1000 rne∆660-1061-cat | This study |
AT18 | MC1000 eno::HA-cat | This study |
AT19 | MC1000 pnp::HA-cat | This study |
AT25 | MC1000 rne::HA-cat | This study |
AT27 | MC1000 rne1-659::HA-kan | This study |
AT28 | MC1000 rne1-417::HA-kan | This study |
AT33 | MC1000 rne::HA | This study |
AT34 | MC1000 rne1-659::HA | This study |
AT35 | MC1000 rne1-417::HA | This study |
AT41 | AT33 ∆minB | This study |
AT43 | AT8 ∆sfiA | This study |
WC1001 | PB103 ftsZ84ts | 9 |
a
Plasmid made in pMLB1113 vectorb
Not viable in the absence of complementing plasmid1. de Boer PAD, Crossley RE, Rothfield LI (1989) Cell 56:641-649.
2. Uzzau S, Figueroa-Bossi N, Rubino S, Bossi L (2001) Proc Natl Acad Sci USA 98:15264-15269.
3. Datsenko KA, Wanner BL (2000) Proc Natl Acad Sci USA 97:6640-6645.
4. Cherepanov PP, Wackernagel W (1995) Gene 158:9-14.
5. Ma L, King GF, Rothfield L (2003) J Bacteriol 185:4948-4955.
6. Rowland SL, Fu X, Sayed MA, Zhang Y, Cook WR, Rothfield LI (2000) J Bacteriol 182:613-619.
7. Wang M, Cohen SN (1994) Proc Natl Acad Sci USA 91:10591-10595.
8. Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL (2000) Proc Natl Acad Sci USA 97:5978-5983.
9. Cook W, Rothfield L (1994) Mol Microbiol 14:485-495.