Supplementary material for Sandre et al. (1999) Proc. Natl. Acad. Sci. USA 96 (19), 10591-10596.

GIF Image

Fig. 2.

(a) Sharp electrode introduced inside an adhering vesicle that contains sucrose and 1 mM NaCl for electrical conductivity. The membrane reseals tightly around the glass pipette, so that the current lines (10 pA) are confined through the lipidic bilayer. (b) Enlargement for another vesicle showing the tip end plugged by a small vesicle.