Supplementary material for Moran et al. (1999) Proc. Natl. Acad. Sci. USA 96 (19), 10699-10704.

Table 3.

 Parameters of crosslinked GCN4-p2¢ folding

 

Species

Equilibrium

Kinetics

-D G°

m°

R

T ln kf

R

T ln ku

- mf

mu

-D G° =

D G‡u - D G‡f

m°

=

mu

 - mf

Wild type

8.9 ± 0.4

1.6 ± 0.1

5.0 ± 0.1

-4.5 ± 0.4

0.79 ± 0.05

0.98 ± 0.06

9.5 ± 0.4

1.77 ± 0.08

D7A

9.75 ± 0.44

1.84 ± 0.08

5.7 ± 0.1

-4.7 ± 0.3

0.78 ± 0.03

1.03 ± 0.06

10.4 ± 0.3

1.81 ± 0.09

D7G

8.1 ± 0.21

1.77 ± 0.05

5.3 ± 0.1

-2.6 ± 0.3

1.11 ± 0.05

0.74 ± 0.05

7.9 ± 0.3

1.85 ± 0.07

S14A

11.4 ± 0.06

1.98 ± 0.01

5.6 ± 0.1

-4.6 ± 0.3

0.83 ± 0.02

0.95 ± 0.05

10.2 ± 0.3

1.78 ± 0.05

A24G

6.5 ± 0.1

1.59 ± 0.02

5.1 ± 0.3

-1.0 ± 0.4

0.81 ± 0.05

0.76 ± 0.08

6.1 ± 0.5

1.57 ± 0.09

AAG

6.8 ± 0.1

1.56 ± 0.02

6.7 ± 0.2

-0.8 ± 0.2

1.14 ± 0.06

0.67 ± 0.04

7.5 ± 0.3

1.81 ± 0.07

GGG

4.4 ± 0.03

1.9 ± 0.01

4.6 ± 0.2

-0.1 ± 0.2

1.3 ± 0.14

0.74 ± 0.07

4.7 ± 0.3

2.04 ± 0.16

TTT

ND

ND

4.8 ± 0.1

-2.7 ± 0.2

0.96 ± 0.03

0.80 ± 0.03

7.5 ± 0.2

1.76 ± 0.03

AAA

11.1 ± 0.1

1.97 ± 0.01

5.8 ± 0.2

-4.8 ± 0.6

0.87 ± 0.04

0.98 ± 0.09

10.7 ± 0.7

1.85 ± 0.10

The DG° values and activation energies (units of kcal/mol) are extrapolated to zero denaturant. Units of m are kcal/mol per M. ND, not determined.