Supplementary material for Jackson et al. (1999) Proc. Natl. Acad. Sci. USA 96 (19), 10875-10880.
Table 4.
Bacterial strains and plasmidsStrain | Relevant characteristics | Source/Ref. |
Pseudomonas syringae pv. phaseolicola | ||
1302A | Race 4 | 1 |
1448A | Race 6 | 2 |
1449B | Race 7, pAV511, pAV512 | 1 |
RW50 | Path-, pAV512,pPPY51, Ap-r | This study |
RW57 | Path-, pAV512, Ap-s | This study |
RW60 | Path-, pAV512, Ap-s, Rif-r | This study |
Escherichia coli | ||
DH5a | F-, recA, D lacU169(F 80 lacZD M15), endA, hsdR, gyrA | GIBCO/BRL |
C2110 | Nal-r, polA- | 3 |
MG1 | Phaseolotoxin-sensitive | M. Gasson, Institute for Food Research, Norwich, U.K. |
MG2 | Phaseolotoxin-resistant | M. Gasson |
39R861 | Hosts four marker plasmids (154 kb, 66.2 kb, 37.6 kb, 7.4 kb) | 4 |
Plasmids | ||
pBluescript II KS+ | Ap-r, ColE1 replicon | Stratagene |
pLAFR3 | Tc-r, Tra-, Mob+, cos, RK2 replicon | 5 |
pBBR1MCS-2 | Km-r, Tra-, Mob+ | M. Kovach, Louisiana State University Medical Center, Shreveport, LA |
pRK2013 | Km-r, Tra+, ColE1 replicon | 6 |
pRK2073 | St-r/Sp-r, Tra+, ColE1 replicon | 7 |
pHoKmGus | Ap-r, Km-r, tnpA-, pWB15A replicon | 8 |
pSShe | Cm-r, tnpA+, pACYC184 | 3 |
pAV505 | Native plasmid from 1302A, ca. 140 kb | 9 |
pAV511 | Native plasmid from 1449B, ca. 154 kb | This study |
pAV512 | Native plasmid from 1449B, ca. 32 kb | This study |
pPPY51 | 4.3-kb PstI fragment harboring pAV505 sequences and oriV-505 from pPPY40 in pBluescript II KS+ | This study |
pAV518 | Genomic cosmid clone (partial Sau3AI in BamHI) harboring pAV511 sequences in pLAFR3 | This study |
pAV521 | Genomic cosmid clone (partial Sau3AI in BamHI) harboring pAV511 sequences and avrPphF in pLAFR3 | This study |
pAV525 | 1.8-kb BamHI fragment harboring avrPphF from pAV521 in pLAFR3 | This study |
pAV530 | 6.7-kb EcoRI fragment harboring virPphA, ORF2, and ORF3 from pAV518 in pLAFR3 | This study |
pAV533 | 3.1-kb EcoRI-Csp45I fragment harboring virPphA from pAV530 in pBBR1MCS-2 | This study |
pAV534 | 3.3-kb EcoRI-Csp45I fragment harboring ORF2 and ORF3 from pAV530 in pBBR1MCS-2 | This study |
pAV535 | 1.6-kb NsiI-FspI fragment harboring ORF4 and part of the Tn501 like tnp from pAV518 in pBBR1MCS-2 | This study |
pAV536 | 1.8-kb NsiI-SspI fragment harboring virPphA in pBBR1MCS-2 | This study |
pAV537 | 1.5-kb Csp45I-SspI fragment harboring ORF2 and ORF3 in pBBR1MCS-2 | This study |
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