Supplementary material for Chang et al. (2000) Proc. Natl. Acad. Sci. USA 97 (8), 4291-4296.

Table 1.

 Genes down-regulated by p21 induction

Genes

Accession no.

Balanced differential expression

Confirmed by*

A. p21-inhibited genes identified by UniGemV array

Associated with mitosis

    

CDC2

X05360

2.5

R, W

    

CKsHs1 (CDC2 kinase)

X54941

5.5

R

    

PLK1 (polo-like kinase)

U01038

5.1

R, W

    

XCAP-H condensin homolog

D38553

6

R

    

CENP-A (centromere protein A)

U14518

5.3

R

    

CENP-F (centromere protein F)

U30872

2.5

R

    

MAD2

U65410

6.6

R, W

    

BUBR1

AF053306

5.9

R

    

MCAK (mitotic centromere-associated kinesin)

U63743

3.8

R

    

HSET kinesin-like protein

AL021366

3.6

R

    

CHL1 helicase

U75968

3.3

R

    

AIK-1 (aurora/IPL1-related kinase)

D84212

4.6

R

    

AIM-1 (AIK-2; aurora/IPL1-related kinase)

AF004022

10.2

R

    

PRC1 (protein regulating cytokinesis 1)

AF044588

12.6

R, W

    

Citron kinase†

H10809

2.7

R

    Lamin B1

L37747

7

 

    

Lamin B2

M94362

2.7

 

    

LAP-2 (lamin-associated protein 2)

U18271

4.6

R

    

MPP2 (M phase phosphoprotein 2)

U74612

3.7

R

    

MPP5 (M phase phosphoprotein 5)

X98261

3.7

 

Associated with DNA replication, segregation and chromatin assembly

    

Thymidine kinase 1

K02581

2.9

R

    

Thymidylate synthase

X02308

3.9

R

    

Uridine phosphorylase

X90858

2.5

 

    

Ribonucleotide reductase M1

X59543

4.6

R

    

Ribonucleotide reductase M2

X59618

10.7

R

    

CDC47 homolog (MCM7)

D55716

9.6

R

    

CDC21 homolog (MCM4)

X74794

2.7

R

    

CDC45 homolog (Porc-Pl)

AJ223728

4.1

R

    

HsORC1 (origin recognition complex 1)

U40152

2.7

R

    

DNA polymerase a

X06745

2.8

R

    Replication factor C (37-kD subunit)

M87339

2.6

 

    

B-MYB

X13293

9.1

 

    

HPV16 E1 protein binding protein

U96131

3.7

 

    

Topoisomerase IIa

J04088

8.6

R

    

Chromatin assembly factor-I (p60 subunit)

U20980

2.7

R

    

High-mobility group chromosomal protein 2

X62534

3.7

R

    

High-mobility group chromosomal protein 1

D63874

3.6

R

    

Histone H2A.F/Z variant

AA203494

2.8

 

Associated with DNA repair

    

XRCC9

U70310

3.6

R

    

RAD54 homolog

X97795

5.4

R

    

HEX1 5'-3' exonuclease (RAD2 homolog)

AF042282

5.2

R

    

ATP-dependent DNA ligase I

M36067

2.5

R

    

RAD21 homolog

D38551

2.9

R

Associated with transcription and RNA processing

    

Putative transcription factor CA150

AF017789

2.8

 

    

Transcriptional coactivator ALY

AF047002

3.3

 

    

WHSC1/MMSET (SET domain protein)

AA401245

2.9

 

    

NN8-4AG (SET domain protein)

U50383

2.8

 

    

EZH2 (enhancer of zeste homolog 2)

U61145

2.8

 

    

PTB-associated splicing factor

X70944

2.5

 

    

AU-rich element RNA-binding protein AUF1

U02019

2.8

 

    

U-snRNP-associated cyclophilin

AF016371

2.8

 

Other genes

    

3-Phosphoglycerate dehydrogenase

AF006043

4.8

 

    

L-type amino acid transporter, subunit LAT1

M80244

4.1

R

    

Hyaluronan-mediated motility receptor

U29343

4

 

    

Phorbolin I (PKC-inducible)

U03891

3.9

 

    

PSD-95 binding family protein

D13633

3.7

R

    

HTRIP (TNF receptor component)

U77845

3.6

 

    

NAD-dependent methylenetetrahydrofolate dehydrogenase

X16396

3.4

 

    

Membrane glycoprotein 4F2 antigen heavy chain

J02939

3.2

 

    

Mucin-like protein

D79992

3.2

 

    

MAC30 (differentially expressed in meningiomas)

L19183

2.9

 

    

P52rIPK (regulator of interferon-induced protein kinase)

AF007393

2.8

 

    

Putative phosphoserine aminotransferase

AA192483

2.8

 

    

Glucose 6-phosphate translocase

Y15409

2.7

 

    

Calcyclin-binding protein

AF057356

2.6

 

    

Ornithine decarboxylase 1

X16277

2.6

R

    

Trophinin assisting protein (tastin)

U04810

2.5

 

    

Acyl-coenzyme A cholesterol acyltransferase

L21934

2.5

 

    

Pinin/SDK3

Y10351

2.5

 

Genes with unknown function

    

EST

AA975298

2.7

 

    

EST

AA034414

2.5

 

    

EST

AA482549

2.5

 

Genes

Accession no.

UniGemV result‡

 

B. p21-inhibited genes identified by RT-PCR

Cyclin A1

U66838

IS

 

Cyclin B1

M25753

IS

 

CDC25A

NM_001789

A

 

Dihydrofolate reductase

J00140

1.5

 

ING1

NM_005537

A

 

*R, RT-PCR; W, Western blotting.

†

The original clone was identified as an EST but was found by database search to map 3' to the coding sequence of the citron kinase gene; inhibition of this gene was then demonstrated by RT-PCR based on the coding sequence of citron kinase.

‡

IS, insufficient signal; A, absent from the array.