Damage-induced neuronal endopeptidase (DINE) is a unique metallopeptidase expressed in response to neuronal damage and activates superoxide scavengers -- Data Supplement - Supplemental Table 1 -- Proceedings of the National Academy of Sciences

Supplementary material for Kiryu-Seo et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.070509897

Table 1. Expression profile of DINE under various kinds of injuries

Damage	Expression	Expression site
Hypoglossal nerve transection	Up-regulated	Hypoglossal nucleus
Facial nerve transection	Up-regulated	Facial nucleus
Sciatic nerve transection	Up-regulated	Dorsal root ganglia
Optic nerve crush	Up-regulated	Ganglion cells
MCA occlusion	Up-regulated	Cortex, thalamus
Cortical and corpus callosum injury	Up-regulated	Cortex, thalamus
Colchicine injection (3 V)	Up-regulated	Hypothalamus, thalamus, cortex
Colchicine injection (tongue)	Up-regulated	Hypoglossal nucleus
Kainate-induced hyperexcitation	No change
Osmotic stress	No change
Immobilization stress	No change

Various kinds of damaged paradigms were performed. Expression of DINE mRNA was examined by in situ hybridization. The rats were killed and analyzed 5 days after injuries including hypoglossal, facial, sciatic, and optic nerve injury; MCA occlusion; and cortical and corpus callosum transection. Colchicine (90 µg) was injected into third ventricle and tongue. Kainate (0.8 µg) was injected into third ventricle. For osmotic stress, 2 M NaCl was injected i.p. These rats were killed 48 hr after stimulation.