Supplementary material for Sutter et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.080072497

Table 1.

 Primers used for PCR

 

Sequence

FP1

RP1

FP2

RP2

A. C-terminal deletion

1-826

187

350

 

 

1-754

187

356

 

 

1-729

187

363

 

 

1-726

187

364

 

 

1-703

187

362

 

 

1-681

187

355

 

 

1-608

187

349

 

 

B. Internal deletions

1-391/404-826

409

350

 

 

1-391/415-826

408

350

 

 

1-391/429-826

407

350

 

 

1-391/469-826

369

350

 

 

1-391/494-826

370

350

 

 

1-391/508-826

376

350

 

 

1-391/512-826

375

350

 

 

1-391/517-826

380

350

 

 

1-391/521-826

371

350

 

 

1-391/549-826

372

350

 

 

1-391/576-826

365

350

 

 

C. Missense mutations

C520M

386

350

 

 

S515A/S517A

377

350

 

 

Y519F

378

350

 

 

P513G/P516G

381

350

 

 

E512G/E518G

382

350

 

 

S551G/T552A

187

385

384

350

S551D/T552D

187

432

431

350

D. GAL4/HIF-1a

Wild type

407

349

 

 

S551G/T552A

407

385

384

349

S551G

407

414

413

349

E. His6-HIF-1a

 

168

999