Participation of mouse DNA polymerase {iota} in strand-biased mutagenic bypass of UV photoproducts and suppression of skin cancer -- Dumstorf et al. 103 (48): 18083 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Dumstorf et al. 10.1073/pnas.0605247103.

Supporting Tables

Files in this Data Supplement:

Supporting Table 2
Supporting Table 3
Supporting Table 4
Supporting Table 5

Table 2. UV-induced mutations at the Hprt locus in primary fibroblasts derived from Pol^+/+ Pol^+/+mice

Mutant	Mutation	Position	Surrounding sequence*	Amino acid changed	Strand with affected dipyrimidine
Dipyrimidine Base Substitutions
Mutations involving cytosine
WT7	C•G ® T•A	74	ATA CCT AAT	Pro ® Leu	Nontranscribed
WT15	C•G ® T•A	74	ATA CCT AAT	Pro ® Leu	Nontranscribed
WT3	C•G ® T•A	74	ATA CCT AAT	Pro ® Leu	Nontranscribed
WT19	C•G ® T•A	113	ATT CCT CAT	Pro ® Leu	Nontranscribed
WT1	C•G ® T•A	145	AGA CTT GCT	Leu ® Phe	Nontranscribed
WT18	C•G ® T•A	145	AGA CTT GCT	Leu ® Phe	Nontranscribed
WT512	C•G ® T•A	145	AGA CTT GCT	Leu ® Phe	Nontranscribed
WT412	C•G ® T•A	151	GCT CGA GAT	Arg ® STOP	Nontranscribed
WT4	C•G ® T•A	209	AAG GGG GGC	Gly ® Glu	Transcribed
WT14	C•G ® T•A	508	TCT CGA AGT	Arg ® STOP	Nontranscribed
WT22	C•G ® T•A	550	ATT CCA GAC	Pro ® Ser	Nontranscribed
WT112	C•G ® T•A	550	ATT CCA GAC	Pro ® Ser	Nontranscribed
WT15	C•G ® T•A	601	AGG GAT TTG	Asp ® Asn	Transcribed

WT40	C•G ® A•T	134	GAC AGG ACT	Arg ® Met	Transcribed
WT35	C•G ® A•T	379	ACT GGA AAG	Gly ® STOP	Transcribed
Mutations involving thymidine
WT44	T•A ® C•G	194	GCC CTC TGT	Leu ® Pro	Nontranscribed
WT6	T•A ® A•T	125	CTG ATT ATG	Ile ® Asn	Nontranscribed
WT51	T•A ® A•T	125	CTG ATT ATG	Ile ® Asn	Nontranscribed
WT7	T•A ® A•T	125	CTG ATT ATG	Ile ® Asn	Nontranscribed
WT28	T•A ® A•T	245	TAC ATT AAA	Ile ® Asn	Nontranscribed
WT8	T•A ® A•T	245	TAC ATT AAA	Ile ® Asn	Nontranscribed
WT532	T•A ® A•T	410	ATA ATT GAC	Ile ® Asn	Nontranscribed
WT213	T•A ® G•C	194	GCC CTC TGT	Leu ® Arg	Nontranscribed
WT512	T•A ® G•C	194	GCC CTC TGT	Leu ® Arg	Nontranscribed
WT10	T•A ® G•C	424	AAA ACA ATG	Thr ® Pro	Transcribed
WT71	T•A ® G•C	449	CTG GTT AAG	Val ® Gly	Nontranscribed
WT510	T•A ® G•C	449	CTG GTT AAG	Val ® Gly	Nontranscribed

Mutant	Mutation	Position	Surrounding sequence*	Amino acid changed	Strand with affected dipyrimidine
Nontandem double base substitutions
WT2	C•G ® T•A	113	ATT CCT CAT	Pro ® Leu
WT2	C•G ® A•T	115	ATT CCT CAT	His ® Asn
WT12	C•G ® T•A	113	CCT CAT GGA	Pro ® Leu
WT12	C•G ® A•T	118	CCT CAT GGA	Gly ® STOP
Nondipyrimidine base substitutions
WT31^†	C•G ® A•T	157	GAT GTC ATG	Val ® Phe
WT43	T•A ® A•T	1	GTC ATG CCG	START ® Leu
Frameshifts
WT83	+T	372 - 374	ACT TTA ACT
WT11	+T	655	AAA GCC TAA	STOP ® Leu
Exon deletions
WT96		Exon 2-3
WT171		Exon 7
Large insertion
WT243	+67 bases	403,404

*Sequences of the non-transcribed (coding) strand.

^†Complex photoproducts have been suggested to occur at ACA sequences.

Table 3. UV-induced mutations at the Hprt locus in primary fibroblasts derived from Pol^-/- Pol^+/+ mice

Mutant	Mutation	Position	Surrounding sequence*	Amino acid changed	Strand with affected dipyrimidine
Dipyrimidine Base Substitutions
Mutations involving cytosine
H6	C•G ® T•A	74	ATA CCT AAT	Pro ® Leu	Nontranscribed
H3	C•G ® T•A	74	ATA CCT AAT	Pro ® Leu	Nontranscribed
H31	C•G ® T•A	464	AGC CCC AAA	Pro ® Leu	Nontranscribed
H5	C•G ® T•A	464	AGC CCC AAA	Pro ® Leu	Nontranscribed
H29	C•G ® T•A	464	AGC CCC AAA	Pro ® Leu	Nontranscribed
H12	C•G ® T•A	544	TTT GAA ATT	Glu ® Lys	Transcribed
H2	C•G ® T•A	551	ATT CCA GAC	Pro ® Leu	Nontranscribed
H15	C•G ® T•A	551	ATT CCA GAC	Pro ® Leu	Nontranscribed
H9	C•G ® T•A	551	ATT CCA GAC	Pro ® Leu	Nontranscribed

H62	C•G ® A•T	329	CAG TCA ACG	Ser ® STOP	Nontranscribed
H18	C•G ® A•T	329	CAG TCA ACG	Ser ® STOP	Nontranscribed
H16	C•G ® A•T	355	GGT GGA GAT	Gly ® STOP	Transcribed
H13	C•G ® A•T	400	GTT GAA GAT	Glu ® STOP	Transcribed
H1	C•G ® A•T	580	CTT GAC TAT	Asp ® Tyr	Transcribed
H17	C•G ® A•T	589	AAT GAG TAC	Glu ® STOP	Transcribed
H23	C•G ® A•T	601	AGG GAT TTG	Asp ® Tyr	Transcribed
H21	C•G ® A•T	606	GAT TTG AAT	Leu ® Phe	Transcribed
H14	C•G ® A•T	634	ACT GGA AAA	Gly ® STOP	Transcribed

H19	C•G ® G•C	550	ATT CCA GAC	Pro ® Ala	Nontranscribed
Mutations involving thymidine
H23	T•A ® A•T	214	GGC TAT AAG	Tyr ® Asn	Nontranscribed
H24	T•A ® A•T	389	AAT GTC TTG	Val ® Asp	Nontranscribed
H71	T•A ® A•T	389	AAT GTC TTG	Val ® Asp	Nontranscribed
H85	T•A ® A•T	437	ACT TTG CTT	Leu ® STOP	Nontranscribed
H45	T•A ® A•T	543	GGA TTT GAA	Phe ® Leu	Nontranscribed

H76	T•A ® C•G	203	GTG CTC AAG	Leu ® Pro	Nontranscribed
H90	T•A ® C•G	203	GTG CTC AAG	Leu ® Pro	Nontranscribed
H53	T•A ® C•G	254	GCA CTG AAT	Leu ® Pro	Nontranscribed
H84	T•A ® C•G	392	GTC TTG ATT	Leu ® Ser	Nontranscribed
H26	T•A ® C•G	404	GAA GAT ATA	Asp ® Gly	Transcribed
H78	T•A ® C•G	437	ACT TTG CTT	Leu ® Ser	Nontranscribed
H30	T•A ® C•G	542	GGA TTT GAA	Phe ® Ser	Nontranscribed

H2	T•A ® G•C	655	AAA GCC TAA	STOP® Glu	Nontranscribed

Tandem dipyrimidine base substitutions
H81	C•G ® T•A	112	ATT CCT CAT	Pro ® Phe	Nontranscribed
H81	C•G ® T•A	113	ATT CCT CAT	Pro ® Phe	Nontranscribed
H20	C•G ® T•A	112	ATT CCT CAT	Pro ® Phe	Nontranscribed
H20	C•G ® T•A	113	ATT CCT CAT	Pro ® Phe	Nontranscribed
Nondipyrimidine base substitutions
H7^†	C•G ® A•T	617	GTT TGT GTC	Cys ® Phe
H22^†	T•A ® A•T	109	TTT ATT CCT	Ile ® Phe
H27^†	T•A ® G•C	584	GAC TAT AAT	Tyr ® Ser
H91^†	T•A ® G•C	584	GAC TAT AAT	Tyr ® Ser
Frameshift mutations
H99	+T	578 - 579	GCC Ctt GAC	Asp ® STOP
H37	-TA	520, 521	GGA TAC AGG
Exon deletions
H25		Exon 2
H10		Exon 2
H8		Exon 2-3
H60		Exon 2-5
H68		Exon 2-7
Deletions
H11^‡		∆403 - 456
H4^‡		∆404 - 448
H93^§		∆533 - 553

*Sequences of the nontranscribed (coding) strand.

^†Complex photoproducts have been suggested to occur at ACA and TGT sequences. ^‡Presumed cryptic splice acceptor within exon 6. ^§Presumed cryptic splice acceptor within exon 8.

Table 4. UV-induced mutations at the Hprt locus in primary fibroblasts derived from Pol^+/+ Pol^-/- mice

Mutant	Mutation	Position	Surrounding sequence*	Amino acid changed	Strand with affected dipyrimidine
Dipyrimidine Base Substitutions
Mutations involving cytosine
I2	C•G ® T•A	113	ATT CCT CAT	Pro ® His	Nontranscribed
I1	C•G ® T•A	209	AAG GGG GGC	Gly ® Glu	Transcribed
I5	C•G ® T•A	325	GAT CAG TCA	Gln ® STOP	Nontranscribed
I42	C•G ® T•A	463	AGC CCC AAA	Pro ® Ser	Nontranscribed
I62	C•G ® T•A	544	TTT GAA ATT	Glu ® Lys	Transcribed
I32	C•G ® A•T	634	ACT GGA AAA	Gly ® STOP	Nontranscribed
Mutations involving thymidine
I24	T•A ® A•T	64	TTG TTT TGT	Phe ® Ile	Nontranscribed
I28	T•A ® A•T	64	TTG TTT TGT	Phe ® Ile	Nontranscribed
I79	T•A ® A•T	125	CTG ATT ATG	Ile ® Asn	Nontranscribed
I17	T•A ® A•T	245	TAC ATT AAA	Ile ® Asn	Nontranscribed
I6	T•A ® A•T	247	AAT AAA GCA	Lys ® STOP	Transcribed
Tandem dipyrimidine base substitutions
I14	C•G ® T•A	171	ATG GGA GGC	Met ® Ile	Transcribed
I14	C•G ® T•A	172	ATG GGA GGC	Gly ® Arg	Transcribed
I22	C•G ® A•T	112	ATT CCT CAT	Pro ® Ile	Nontranscribed
I22	C•G ® T•A	113	ATT CCT CAT	Pro ® Ile	Nontranscribed
I12	T•A ® A•T	389	AAT GTC TTG	Val ® Asp	Nontranscribed
I12	C•G ® T•A	390	AAT GTC TTG	Val ® Asp	Nontranscribed
Nontandem double base substitutions
I8	C•G ® A•T	589	AAT GAG TAC	Glu ® STOP
I8	C•G ® T•A	591	AAT GAG TAC	Glu ® STOP
I91	C•G ® T•A	599	TTC AGG GAT	Arg ® Lys
I91	C•G ® T•A	601	TTC AGG GAT	Asp ® Asn
Nondipyrimidine base substitutions
I3^†	C•G ® A•T	397	ATT GTT GAA	Val ® Phe
I161	C•G ® A•T	482	GTT GCA AGC	Ala ® Glu
Exon deletions
I412		Exon 2-3

*Sequences of the non-transcribed (coding) strand

^†Complex photoproducts have been suggested to occur at ACA sequences.

Table 5. UV-induced mutations at the Hprt locus in primary fibroblasts derived from Polh^-/- Poli^-/- mice

Mutant	Mutation	Position	Surrounding sequence*	Amino acid changed	Strand with affected dipyrimidine
Dipyrimidine Base Substitutions
Mutations involving cytosine
HI761^†	C•G ® T•A	34	AGC GAT GAT	Asp ® Asn	Transcribed
HI757	C•G ® T•A	325	GAT CAG TCA	Gln ® STOP	Nontranscribed
HI7	C•G ® T•A	464	AGC CCC AAA	Pro ® Leu	Nontranscribed
HI759^†	C•G ® T•A	493	CTG GTG AAA	Val ® Met	Transcribed
HI81	C•G ® A•T	538	GTT GGA TTT	Gly ® STOP	Transcribed
HI61	C•G ® A•T	606	GAT TTG AAT	Leu ® Phe	Transcribed
HI759^†	C•G ® A•T	606	GAT TTG AAT	Leu ® Phe	Transcribed
HI761^†	C•G ® A•T	606	GAT TTG AAT	Leu ® Phe	Transcribed
Mutations involving thymidine
HI76	T•A ® C•G	146	AGA CTT GCT	Leu ® Pro	Nontranscribed
HI761^†	T•A ® C•G	203	GTG CTC AAG	Leu ® Pro	Nontranscribed
HI10^†	T•A ® C•G	194	GCC CTC TGT	Leu ® Pro	Nontranscribed
HI4	T•A ® A•T	247	ATT AAA GCA	Lys ® STOP	Transcribed
HI11	T•A ® A•T	543	GGA TTT GAA	Phe ® Leu	Nontranscribed
HI10^†	T•A ® A•T	656	GCC TAA GAT	STOP ® Leu	Transcribed
Tandem dipyrimidine base substitutions
HI63	C•G ® T•A	112	ATT CCT CAT	Pro ® Phe	Nontranscribed
		113	ATT CCT CAT	Pro ® Phe	Nontranscribed
Tandem non-dipyrimidine base substitutions
HI86	T•A ® G•C	516	AGT GTT GGA	Val ® Val
	C•G ® A•T	517	AGT GTT GGA	Gly ® STOP
Nondipyrimidine base substitutions
HI2^‡	C•G ® A•T	617	GTT TGT GTC	Cys ® Phe
HI759^†,‡	C•G ® T•A	243	GAT TAC ATT	Tyr ® Tyr
HI62^‡	T•A ® A•T	109	TTT ATT CCT	Ile ® Phe
Frameshifts
HI6	∆A	451,452	GTT AAG CAG
Exon deletions
HI83		Exon 2-3
HI32		Exon3
HI114		Exon 4
HI9		Exon 5
HI82		Exon 8

*Sequences of the nontranscribed (coding strand).

^†Mutants containing multiple dimer sites at greater than 150 nucleotides apart. ^‡Complex photoproducts have been suggested to occur at ACA and TGT sequences.