Supporting Figure

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Fig. 7. Cytosolic translocation of PAR polymer after PARP-1 activation. Representative confocal images of cortical neurons after 60 min of NMDA receptor stimulation. DIC images are provided to illustrate neurons. Immunocytochemical staining indicates that PAR polymer (red) not only exists in the nucleus (blue) but also in the cytoplasm in NMDA (500 mM) treated neurons (Lower). Note the thin rim of red cytoplasmic PAR immunoreactivity next to the pink nuclear staining. In CSS-treated neurons, no PAR polymer formation is seen. These experiments have been replicated in separate experiments at least three times with similar results. (Scale bar, 10 mm.)