Supporting Figure

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Fig. 5. Specificity of antibodies raised against S. tuberosum mitochondrial VDAC, TOM20, and TOM40. (A) S. tuberosum mitochondrial proteins from outer membrane analyzed by Western blot according to standard procedures and using antibodies directed against VDAC (lane 1), TOM20 (lane 2), and TOM40 (lane 3). (B) In vitro import of labeled GluRS targeting sequence fused to GFP incubated with isolated mitochondria in the absence (-) or presence (+) of 5 ml of antibodies against VDAC or TOM40. In, input protein (one-tenth of labeled in vitro-synthesized protein).