RNA diversity has profound effects on the translation of neuronal nitric oxide synthase -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Wang et al. (1999) Proc. Natl. Acad. Sci. USA 96 (21), 12150-12155.

Fig. 6.
Tissue-specific expression of the human nNOS exon 1 variants assessed with RNase protection assay. Antisense riboprobes corresponding to varied 5¢ ends of human nNOS (exons 1c, 1f, and 1g) were hybridized to total cellular RNA (50 m g) isolated from human skeletal muscle (SkM), cerebellum (Cer), or yeast tRNA (not shown) and digested with RNase. Protected fragments were size-fractionated alongside RNA markers on denaturing polyacrylamide gels. Nucleotide lengths are indicated (left). Shown are representative results from one of three experiments. Assays used various exon 1-specific cRNA probes, each representing portions of exon 1 as well as exon 2. Protected fragments correspond to unique exon 1/exon 2 species, common exon 2 regions, and/or portions of exon 1 if an nNOS mRNA transcript had undergone a 5¢ alternative splicing event.