Correction of prototypic ATM splicing mutations and aberrant ATM function with antisense morpholino oligonucleotides -- Du et al. 104 (14): 6007 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Du et al. 10.1073/pnas.0608616104.

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SI Figure 7
SI Figure 8

SI Figure 7A
SI Figure 7B

Fig. 7. Real-time RT-PCR. (A) Bars depict the ratios of quantified transcript levels for treated and untreated cells, comparing mRNA levels for mutant ATM to GAPDH, 24 h after incubation with 50 mM cognate AMO. (B) Mutant ATM/GAPDH ratios for transcripts from TAT[C] cells harvested at various time points after incubation with 30 mM of TAT[C]-AMO. Note maximal suppression of mutant ATM transcripts at 48 h.

SI Figure 8A
SI Figure 8B

Fig. 8. Western blots showing time course of restored ATM protein levels following incubation with 30 mM of cognate AMO. (A) IRAT9 cells. (B) AT203LA cells. NBS1 protein was utilized as a loading control. The robust correction of ATM levels after 84 h, for AT203LA cells, was also observed for TAT[C] cells (see Fig. 3D).