Scaffold proteins may biphasically affect the levels of mitogen-activated protein kinase signaling and reduce its threshold properties -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Levchenko et al. (2000) Proc. Natl. Acad. Sci. USA 97 (11), 5818-5823.

Table 1.
Parameter values used in the model (unless otherwise stated)

Parameter	Value assumed^*
Concentrations, mM
[MAPKKK]	0.3
[MAPKK]	0.2
[MAPK]	0.4
[MAPKKK K-ase]	0.2
[MAPKKK P-ase]	0.3
[MAPKK P-ase]	0.2
[MAPK P-ase]	0.3
Association rate constants, m M^-1·sec^-1
a₁	1
a₂	0.5
a₃	3.3
a₄	10
a₅	3.3
a₆	10
a₇	20
a₈	5
a₉	20
a₁₀	5
on₁	10
on₂	10
Dissociation rate constants, sec^-1
d₁	0.4
d₂	0.5
d₃	0.42
d₄	0.8
d₅	0.4
d₆	0.8
d₇	0.6
d₈	0.4
d₉	0.6
d₁₀	0.4
off₁	0.05
off₂	0.05
off₃	0.05
off₄	0.5
Reaction rate constants, sec^-1
k₁	0.1
k₂	0.1
k₃	0.1
k₄	0.1
k₅	0.1
k₆	0.1
k₇	0.1
k₈	0.1
k₉	0.1
k₁₀	0.1

*The concentrations and individual a, d, and k values correspond to estimates in reports (see refs. 26 and 28 of the main paper).