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Fig. 7. Comparison of Ikka sequences in exon 15 from human SCC (Upper) and a subclone in the pGEM-T vector (Lower). An arrow on the top of DNA sequence indicates the sequence containing a single-nucleotide polymorphism. 551 (A to G), an Ikka mutation.

Fig. 8. (Upper) Immunofluorescent staining for HA in skin of WT and Lori•IKKa-Tg (Tg) mice. Red, HA staining; blue, DAPI nuclear staining. (Lower) Immunofluorescent staining for IKKa in the skin of WT and Ikka^-/- (-/-) mice. Green, IKKa staining; blue, DAPI nuclear staining. (Original magnification, ´200.)

Fig. 9. Histology of papillomas and carcinomas induced by treatment with DMBA/TPA and DMBA/DMBA in WT and Lori•IKKa-Tg mice. (A) Histology of papillomas and carcinomas stained with H&E. Magnification is shown at left. (B) Histology of metastatic carcinomas in the lungs. Carcinomas 1-3 were obtained from WT mice treated with DMBA/TPA, and carcinoma 4 was obtained from a WT mouse treated with DMBA/DMBA. Dark brown color, K5-positive staining; blue color, hematoxylin counterstaining; K5, immunohistochemical staining. Magnification is shown at left.

Fig. 10. Histology of well differentiated (Left) and poorly differentiated (Center) carcinomas stained with H&E and immunohistochemical-stained with antibodies against K5, K10, and filaggrin. Skin specimens from the same paraffin section stained with anti-K10 and filaggrin antibodies were used as positive controls for the poorly differentiated carcinomas (Cont; Right). Dark brown, positive staining; blue, nuclear counter staining. (Original magnification: ´200.)

Fig. 11. Immunofluorescent staining for VEGF-A in the skin of WT and Lori•IKKa-Tg (Tg) mice. Red, VEGF-A staining; blue, DAPI nuclear staining. Ep, epidermis between the two arrows. White lines separate dermis and epidermis. (Original magnification: ´200.)