A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Smith et al. (2000) Proc. Natl. Acad. Sci. USA 97 (12), 6658–6663.

Table 2. Yeast strains

Strain	Genotype
JS218	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15 sir2D::HIS3
JS311	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15 RDN1::mURA3-HIS3
M217	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15 RDN1::mURA3-HIS3 npt1-1
M269	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15 RDN1::mURA3-HIS3 npt1-2
JS325	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15
JS596	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15 npt1D::kanMX
JS597	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15 npt1D::kanMX
JS566	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 RDN1::Ty1-MET15, RDN1::mURA3-HIS3 sir2D::kanMX4
YCB647	MATa his3 D200 leu2D1 lys2D202 ura3-52 leu2D::TRP1 ADH4::URA3-TEL
YCB652	MATa his3 D200 leu2D1 lys2D202 ura3-52 ADH4::URA3-TEL sir2D2::TRP1
YCB172	MAT a ura3-52 his3 D 200 leu2 D1 trp1D63 ade2-101 lys2-801hst2D1::TRP1
YCB178	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 sir2D1::URA3
YCB180	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 (=YPH500)
YCB 234	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 hst1D2::LEU2 hst2D1::TRP1
YCB232	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 hst1D2::LEU2
YCB394	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 hst3D3::HIS3
YCB496	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 hst1D2::LEU2 hst2D1::TRP1 hst3D3::HIS3 hst4D1::URA3 sir2D1::URA3
YCB497	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 hst1D2::LEU2 hst2D1::TRP1 hst3D3::HIS3 hst4D1::URA3 sir2D1::URA3
YCB526	MAT a ura3-52 his3D200 leu2D1 trp1D63 ade2-101 lys2-801 hst4D1::URA3
JS641	MATa his3 D200 leu2D1 lys2D202 ura3-52 leu2D::TRP1 ADH4::URA3-TEL npt1D::kanMX4
JS642	MATa his3 D200 leu2D1 lys2D202 ura3-52 leu2D::TRP1 ADH4::URA3-TEL npt1D::kanMX4
YCB761	MATa ade2-101 his3 D200 leu2D1 lys2-801 trp1D63 ura3-52 ADE2-TEL
YCB762	MATa ade2-101 his3 D200 leu2D1 lys2-801 trp1D63 ura3-52 sir2D1::URA3 ADE2-TEL
JS692	MATa ade2-101 his3 D200 leu2D1 lys2-801 trp1D63 ura3-52 npt1D::kanMX4 ADE2-TEL
YL559*	MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrDA::TRP1
MC119*	MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrDA::TRP1 sir2::HIS3
JS643	MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrDA::TRP1 npt1D::kanMX4
JS644	MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hmrDA::TRP1 npt1D::kanMX4
JS646	MAT a his3D200 leu2D1 met15D0 trp1D63 ura3-167 npt1-1

*Provided by David Shore, University of Geneva.