Table 1.
Oligonucleotide primers used for this study Oligonucleotides | Sequence | Position* |
S-1 | 5’-GAATTCAAAACATTCCTATGTGGAGC-3’ | 1895-1920 |
S-1.1 | 5’-CACCATGAATTCAAAGCATTCC-3’ | 1889-1910 |
S-2 | 5’-GCTCCTAACACTCAACTCACT-3’ | 2141-2161 |
S-5.1 | 5’-CAGCAGATCTGAATTGCTGACCTAGATATGG-3’ | 4849-4869 |
S-5.2 | 5’-CAGCAGATCTGATATTGTTAGCGATAAGGCG-3’ | 4884-4904 |
S-5.3 | 5’-CAGCAGATCTGAGGGGACTGGGTATTCGAGT-3’ | 4918-4938 |
S-6.1 | 5’-CAGCAGATCTTACGGTGAACGGTTGGAGAG-3’ | 4965-4984 |
S-11 | 5’-GTCCAGGGGGCCCTTTGGGAACAAG-3’ | 4278-4302 |
S-20 | 5’-GTCGTCTAGACTCAACTCACTATCCGTGTAC-3’ | 2150-2171 |
A-2 | 5’-CGGATCTCAGGGAAGTACCATGAC-3’ | 4585-4562 |
A-2.2 | 5’-CTAATCTAGACGTACGACAGCAGGGCTTGG-3’ | 4662-4643 |
A-2.3 | 5’-GTATTCTAGACTTGTCACAGCCTGGGAGCAG-3’ | 4765-4743 |
A-2.4 | 5’-GGGATCTAGATCTAGGTCAGCAATTCTTAG-3’ | 4865-4845 |
A-2.5 | 5’-TGCGTCTAGACTGCATTGTACTCGAATACCCAG-3’ | 4947-4925 |
A-2.6 | 5’-CACGTCTAGAAGCGTCCATCGATTAGAGCA-3’ | 5040-5021 |
A-9 | 5’-GCCCGAAATATTGACAGGAGG-3’ | 4690-4670 |
A-10 | 5’-CGTGACCCGTCGACCATTCGGATCTC-3’ | 4603-4578 |
A-12 | 5’-GCATAATTCAGGAATGGCAAATACGGAC-3’ | 4484-4457 |
A-25 | 5’-CCGCGGATCCCTCGCATTTTTTATGGACTTG-3’ | 4780-4760 |
A-28 | 5’-CAGCAGATCTAATTCGAGCTCGGTACCCGG-3’ | |
A-32 | 5’-GTCACTGCAGTCGACCATCCGGATCTCAGG-3’ | 4595-4575 |
RVA-1 | 5’-GAGCTTTAAATCTCTGTAGGTAG-3’ | |
S1-FF | 5’-GACTCTGCAGCACCCCAACGGAAAATG-3’ | 2379-2395 |
S1-RF | 5’-TCGTCTAGACTACTTGTCATCGTCGTCCTTGTAATCTGGACTTGTCACAGCCTG-3’ | 4767-4750 |
RACE-A1 | 5’-AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT-3’ | |
RACE-A2 | 5’-TGGAAGAATTCGCGGCCGCAG-3’ | |
RACE-S3 | 5’-GTCATGGTACTTCCCTGAGATC-3’ | 4568-4592 |
*Nucleotide numbers of BDV strain V genome (antigenome polarity). Locations of DNA linker sites are underlined.