Blood, Vol. 109, Issue 10, 4313-4319, May 15, 2007

Macrophage glucocorticoid receptors regulate Toll-like receptor 4–mediated inflammatory responses by selective inhibition of p38 MAP kinase
Blood Bhattacharyya et al. 109: 4313

Supplemental materials for: Bhattacharyya et al, Vol 109, Issue 10, 4313-4319

Files in this Data Supplement:

• Figure S1. Deletion of GR in MGRKO mice (JPG, 56.4 KB) -
  (A) Southern-blot analysis showing disruption of GR in spleen (S) and thioglycollate-elicited macrophages (M) but not in kidney (K) or liver (L). (B) Immunoblot analysis for GR in control and MGRKO macrophages and the effect of Dex on GR expression. Representative of 3 independent experiments.
  
  
  
  
  
  
• Figure S2. Temporal analysis for Dex-dependent suppression of proinflammatory cytokines (JPG, 49.1 KB) -
  Effect of Dex on LPS-mediated induction of TNF- and IL-6 in control macrophages for the indicated periods of time. Values are means ± SEM; n = 2.
  
  
  
  
  
  
• Figure S3.Temporal regulation of p38 MAPK and JNK in LPS-treated macrophages (JPG, 112 KB) -
  (A) Mouse corticosterone (Cort) suppresses LPS-mediated activation of p38 MAPK. Effect of Cort on LPS-induced phosphorylation of p38 MAPK in control and MGRKO macrophages. (B) Temporal analysis for LPS-mediated induction of JNK (p46/p54). Control macrophages were stimulated with LPS for the indicated periods of time. Representative of 2 independent experiments.
  
  
  
  
  
  
• Figure S4. p38 MAPK inhibitors impair proinflammatory cytokine secretion in MGRKO macrophages (JPG, 59 KB) -
  Effect of SB (20 µM) and PD (20 µM) on LPS-mediated induction of TNF- and IL-6. Shown are the mean and SEM of triplicate samples. Representative of 2 independent experiments.
  
  
  
  
  
  
• Figure S5. p38 MAPK inhibitor impairs COX-2 expression in MGRKO macrophages (JPG, 48.5 KB) -
  Effect of SB (20 mM) on LPS-mediated induction of COX-2 for the indicated periods of time. Representative of 2 independent experiments.