DeJong et al. 10.1073/pnas.0608407104. |
Table 2. Primer information
Primer name | Purpose | Sequence (5'-3') |
CATex1F2 | Catalase exon 1 amplification | CAAATCGTTGACTCCAGTGTGCCAG |
CATex1R2 | Catalase exon 1 amplification | CACGGTCGAATTGGTCGACACTG |
CATex2F3 | Catalase exon 2 amplification | TGTGTGTTTTCGTTCTCATA |
CATex2R2 | Catalase exon 2 amplification | CTACTTCCGCGTCGATCAT |
CATEX1-SN1AF | SNP assay primer | TGAAACGGAAAACAAATCCCATCAG |
CATEX1-SN1AR | SNP assay primer | GTATGCGTTCAGCTGGTTTTCG |
CATEX1-SN1AV1 | SNP assay probe (VIC labeled) | AATGTGGCGCAATC |
CATEX1-SN1AM1 | SNP assay probe (FAM labeled) | AATGTCGCGCAATC |
Cat-Ag-AMC+ | qPCR, catalase | GGATAAGGTGACGGCTACGA |
Cat-Ag-AMC- | qPCR, catalase | GAGAAGCGTACGGCTAGTGG |
S7-Ag-qPCR-AMC+ | qPCR, S7 ribosomal protein | AGAACCAGCAGACCACCATC |
S7-Ag-qPCR-AMC- | qPCR, S7 ribosomal protein | GCTGCAAACTTCGGCTATTC |
CAT-Exp-Ser-F | Ser allele for recombinant protein | ATGTGGCGCAATCCGGCCGAAAAC |
CAT-Exp-Trp-F | Trp allele for recombinant protein | ATGTCGCGCAATCCGGCCGAAAAC |
CAT-Exp-R | Recombinant proteins | CAGATTGGCCGTGCGGCGCAGTTT |
Cfw | Catalase fragment for dsRNA | GCAACAATACGCCCATCTTCTTCA |
Crev | Catalase fragment for dsRNA | GGAATGAGCGGGAATTCGTT |
LacZ-FW | LacZ fragment for dsRNA | GAGTCAGTGAGCGAGGAAGC |
LacZ-Rev | LacZ fragment for dsRNA | TATCCGCTCACAATTCCACA |
Xho-T7-M13For | Amplification of cloned fragments for dsRNA | CTCGAGTAATACGACTCACTATAGGGCTAGTAACGGCCGCCAGTGT |
Xho-T7-M13Rev | Amplification of cloned fragments for dsRNA | CTCGAGTAATACGACTCACTATAGGGGCCAGTGTGATGGATATCTGC |
Bolded and underlined nucleotides identify differences for the nonsynonymous SNP resulting in either serine or tryptophan. qPCR, quantitative PCR.