From the Cover: Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis -- Nakazawa et al. 104 (7): 2425 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Nakazawa et al. 10.1073/pnas.0608167104.

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Fig. 7. Deletion of the MCP-1 gene prevents ONL degeneration after RD. (A-D) H&E-stained sections through the retinas of WT mice (A and B) or MCP-1^-/- mice (C and D), either without RD (A and C) or after 7 days of RD (B and D). (Scale bar: 100 mm.) (E) Low-power photograph showing H&E-stained sections 7 days after inducing RD in one-half of the retina. Whereas the left side of the retina remains attached (RD-), the right side is detached (RD+). (Scale bar: 1 mm.) (F) Quantification of ONL thickness in the indicated regions of the retina in WT and MCP-1^-/- mice. *, P < 0.05 compared with WT controls without RD (n = 8 each).

Fig. 8. CD11b⁺ cells in close apposition of MCP-1⁺ cells in the retina. (A-F) IHC for with MCP-1 (A and D), CD11b (B and E), and overlay of the two (C and F) in the OPL with (D-F) or without (A-C) RD at 72 h. (Scale bar: 100 mm.) (G) Confocal photomicrograph (red, MCP-1; green, CD11b) in detached retina at 72 h. (Scale bar: 10 mm.) (H) Overlay photograph showing immunoreactivity for CD11b (green), TUNEL (red), and DAPI staining (blue) in detached retina after 72 h. (Scale bar: 10 mm.) (I) Time course showing the temporal correlation of CD11b⁺ cells in the OPL (diamonds) per fields (´20 objective) and the TUNEL⁺ cells (bar chart) in detached retina (n = 6 each).

Fig. 9. Deletion of the Mac-1 gene suppresses the infiltration of macriphage/microglia after RD. (A-D) IHC with an antibody against CD45, a marker of leukocytes, in untreated mice (A and B) or 72 h after RD (C and D). Panels show WT mice (A and C) or Mac-1^-/- mice (B and D). Arrows indicate CD45⁺ cells. (Scale bar: 50 mm.) (E and F) Quantification of CD45⁺ cells in the OPL (E) and the subretinal space (F). *, P < 0.05 compared to WT mice with RD (n = 8 each).

Fig. 10. Deletion of the Mac-1 gene prevents RD-induced degeneration of the ONL. (A-D) H&E-stained sections through the retina with (B and D) or without (A and C) RD in WT mice (A and B) or Mac-1^-/- mice (C and D) 7 days after RD. (Scale bar: 50 mm.) (F) Quantification of ONL thickness. *, P < 0.05 compared to WT mice without RD (n = 8 each).