The promyelocytic leukemia protein PML regulates c-Jun function in response to DNA damage -- Salomoni et al. 105 (9): 3686 Data Supplement - Supplemental materials for: Salomoni et al, Vol 105, Issue 9, 3686-3690 -- Blood

Blood, Vol. 105, Issue 9, 3686-3690, May 1, 2005

The promyelocytic leukemia protein PML regulates c-Jun function in response to DNA damage
Blood Salomoni et al. 105: 3686

Supplemental materials for: Salomoni et al, Vol 105, Issue 9, 3686-3690

Files in this Data Supplement:

Figure S1. Cisplatin-induced apoptosis is reduced in Pml^-/- MEFs (JPG, 64.6 KB) -
Analysis of apoptosis in Pml^+/+ and Pml-/- MEFs treated with 2.5 or 5.0 µg/mL cis-pt by subdiploid peak analysis. Average of 3 independent experiments ± SD is shown.
Figure S2. c-Jun DNA-binding activity is impaired in Pml^-/- MEFs (JPG, 57.8 KB) -
Pml+/+ and Pml^-/- MEFs were harvested 0 and 2 hours after UV irradiation (60 J/m²). Nuclear extracts were tested for DNA binding activity to a jun2 CRE site. Supershift experiments were performed by using antibodies against c-Jun and ATF-2. Sp1 binding activity was measured as control of loading. Data shown are representative of 3 independent experiments.
Figure S3. Colocalization of P-c-Jun and PML is statistically significant (JPG, 261 KB) -
Micrographs shown in Figure 5A were analyzed using the ImarisColoc software (Bitplane, Zurich, Switzerland). Statistical analysis is shown.