Identification and characterization of circulating human transitional B cells -- Sims et al. 105 (11): 4390 Data Supplement - Supplemental materials for: Sims et al, Vol 105, Issue 11, 4390-4398 -- Blood

Identification and characterization of circulating human transitional B cells
Blood Sims et al. 105: 4390

Supplemental materials for: Sims et al, Vol 105, Issue 11, 4390-4398

Files in this Data Supplement:

Figure S1. BAFF enhances survival and Ig production of human spleen B cells (JPG, 78 KB) -
(A) Purified B cells or sorted T1 B cells were cultured for 1, 3 or 6 days with various concentrations of BAFF and the percentage cell survival was determined by flow cytometry using forward/side scatter characteristics and 7-AAD exclusion. (B) Human spleen B cells were enriched by negative selection and cultured with various stimuli for 3, 6 and 9 days and cell survival was determined. (C) Ig production was determined from splenic B cell supernatants after 6 days of culture by ELISA. SAC is Staphlococcus aureus Cowan I strain.
Figure S2. T1 B cell Ig heavy chain repertoire (JPG, 125 KB) -
The frequency of V, D and J gene segments was determined for productive V-D-J rearrangements from the combined T1 B cell population isolated from bone marrow (n=26), and peripheral blood from a normal donor (n=23) and an SLE patient (n=21).