HTLV-1 Tax transgenic mice develop spontaneous osteolytic bone metastases prevented by osteoclast inhibition -- Gao et al. 106 (13): 4294 Data Supplement - Supplemental materials for: Gao et al -- Blood

HTLV-1 Tax transgenic mice develop spontaneous osteolytic bone metastases prevented by osteoclast inhibition
Blood Gao et al. 106: 4294

Supplemental materials for: Gao et al

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Figure S1. One month old Tax+ mice show normal osteoclasts both in vivo and in vitro (PDF, 1.96 MB) - A. Osteoclasts cultured from bone marrow macrophages were evaluated for production of the osteoclast specific marker TRAP. No difference in TRAP production was observed between wild type and Tax⁺ osteoclasts from one month old pre-leukemic mice (p>0.05). All data are depicted as the mean+/- s.e.m. B. RT-PCR of bone marrow osteoclasts from Tax⁺ mice failed to express granzyme B (the promoter driving Tax⁺ in this animal model), whereas purified bone marrow derived murine NK cells expressed granzyme B. C. Histomorphometric analysis on TRAP stained decalcified tibias reveals normal osteoclast numbers per osteoblast surface (OB) in Tax⁺ mice at one month of age compared to wild type littermates. All data are depicted as the mean+/- s.e.m. (p>0.05).
Figure S2. Isolated Tax tumor cells express RANK but not OPG (PDF, 82.8 KB) - RT-PCR for RANK and OPG was performed using single cell suspensions of two Tax⁺ tumors from two separate mice which were sorted by FACS according to FcR II/III (CD16/32) FITC negative (N), low and high (H) intensity. GAPDH was used as a loading control.