Jak3 negatively regulates dendritic-cell cytokine production and survival
Blood Yamaoka et al. 106: 3227

Supplemental materials for: Yamaoka et al

Files in this Data Supplement:

• Figure S1. Decreased splenocytes in Jak3^-/- and γc^-/- mice (JPG, 47.3 KB) -

  

  Spleens were harvested from wild-type, Jak3^-/- and c^-/- mice and the total number of cells was counted after lysis of red blood cells with ACK lysing buffer. The absolute number (mean +/- SD) of splenocytes in wild-type, Jak3^-/- and c^-/- mice from three separate experiments are shown.

• Figure S2. Normal development of Jak3^-/- bone marrow in vivo and in vitro (JPG, 169 KB) -

  

  A) Bone marrow cells from wild-type (left panel) and Jak3^-/- (right panel) mice were cultured for 10 days with Flt3L (100ng/ml). The resultant cells were stained with anti-CD11c antibody. Shaded histograms represent samples stained with an isotype control. The data are representative of 2 separate experiments. B, C) Bone marrow cells from wild-type (CD45.1⁺) and Jak3^-/- (CD45.2⁺) mice were mixed in a 1:1 ratio and injected intravenously into lethally irradiated Rag2^-/- C57BL/6 congenic mice (CD45.1⁺). Six to eight weeks later, low density splenic cells were stained and gated on CD11c⁺ (B)or CD11b⁺ (C) cells to analyze the repopulation with each source.