JAZ mediates G1 cell-cycle arrest and apoptosis by positively regulating p53 transcriptional activity -- Yang et al. 108 (13): 4136 Data Supplement -

Supplemental materials for: Yang et al, Vol 108, Issue 13, 4136-4145 -- Blood

Blood, Vol. 108, Issue 13, 4136-4145, December 15, 2006

JAZ mediates G₁ cell-cycle arrest and apoptosis by positively regulating p53 transcriptional activity
Blood Yang et al. 108: 4136

Supplemental materials for: Yang et al, Vol 108, Issue 13, 4136-4145

Files in this Data Supplement:

Figure S1. The JAZ antibody (JAZ111) can specifically immunoprecipitate and immunoblot FLAG-JAZ (JPG, 53.9 KB) -
FLAG-JAZ/pcDNA3 (empty vector pcDNA3 as a control) was transfected into p53^−/− MEFs for 24 hours. The rabbit JAZ antibody (JAZ111 and the control antibody, as described in “Materials and methods”) was used to immunoprecipitate FLAG-JAZ from 100 µg cell lysate, followed by immunoblotting using JAZ111 and FLAG antibody. 50% input indicates 50 µg cell lysate; +, JAZ111; −, the control Ab; V, pcDNA3; F-J, FLAG-JAZ/pcDNA3; and IgG and Igg, bands of the heavy and light chains of rabbit IgGs, respectively.
Figure S2. RNase V1 is able to efficiently cleave dsRNA in vitro (JPG, 42.9 KB) -
A-130 bp P³²-labeled dsRNA probe was prepared as described³² and then incubated with 0, 0.01, 0.1, or 1 U/mL RNase V1 at room temperature for 1 hour. RNase A (100 µg/mL) that degrades single-stranded RNA but not dsRNA was also included in the solutions to verify the specificity of dsRNA digestion. The samples were then analyzed by 8% native polyacrylamide gel electrophoresis followed by autoradiography.