EphB2 and EphB4 receptors forward signaling promotes SDF-1-induced endothelial cell chemotaxis and branching remodeling -- Salvucci et al. 108 (9): 2914 Data Supplement - Supplemental materials for: Salvucci et al -- Blood

Blood, Vol. 108, Issue 9, 2914-2922, November 1, 2006

EphB2 and EphB4 receptors forward signaling promotes SDF-1–induced endothelial cell chemotaxis and branching remodeling
Blood Salvucci et al. 108: 2914

Supplemental materials for: Salvucci et al

Files in this Data Supplement:

Video 1. Video microscopic documentation of extracellular matrix-dependent cord formation by endothelial cells (MOV, 4.9 MB) -
HUVEC were dispersed on Matrigel-coated chambers in complete culture medium and incubated for 16 hr at (37°C and 5% CO2) on the stage of an inverted confocal scanning microscope. Time-lapse microscopy recorded images at 5 min intervals over 13-14 hr; one second of movie corresponds to approximately 30 min of real time.
Video 2. Defective endothelial cell cord formation in the presence of a CXCR4 inhibitor (MOV, 4.4 MB) -
HUVEC were dispersed onto Matrigel-coated chambers in complete culture medium supplemented with the CXCR4 peptide inhibitor CTCE9908 (50µg/ml), and incubated for 16 hr at (37°C and 5% CO2) on the stage of an inverted confocal scanning microscope. Time-lapse microscopy recorded images at 5 min intervals over 13-14 hr; one second of movie corresponds to approximately 30 min of real time.
Video 3. Impaired endothelial cell cord formation in the presence of a peptide inhibitor of EphB2 (MOV, 4.8 MB) -
HUVEC were dispersed onto Matrigel-coated chambers in complete culture medium supplemented with the EphB2 peptide inhibitor SNEW (100 µM), and incubated for 16 hr at (37°C and 5% CO2) on the stage of an inverted confocal scanning microscope. Time-lapse microscopy recorded images at 5 min intervals over 13-14 hr; one second of movie corresponds to approximately 30 min of real time.
Video 4. Reduced endothelial cell cord formation in the presence of a peptide inhibitor of EphB4 (MOV, 4.76 MB) -
HUVEC were dispersed onto Matrigel-coated chambers in complete culture medium supplemented with the EphB4 peptide inhibitor TNYL-RAW (100 µM), and incubated for 16 hr at (37°C and 5% CO2) on the stage of an inverted confocal scanning microscope. Time-lapse microscopy recorded images at 5 min intervals over 13-14 hr; one second of movie corresponds to approximately 30 min of real time.