Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast -- Data Supplement - Supplemental Figure -- Proceedings of the National Academy of Sciences

Supplementary material for Cooper et al. (December 12, 2000) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.250351297

Fig. 7.
Ultrastructual analysis of ama1D mutants. Wild-type (A and B) and ama1D mutant (C and D) cultures were fixed 24 h following shift to sporulation medium as described [Neiman, A. M. (1998) J. Cell Biol. 140, 29–37] to enhance visualization of the spore wall layers and membranes. The wild-type spore wall contains the inner spore wall (open arrow), two chitosan/chitin layers that appear as a single layer (Ch), and the electron-dense dityrosine outer layer (filled arrow). The putative prospore wall in the mutant (large arrows) and dark staining cytoplasmic vesicles (small arrows) are indicated in the ama1D mutant. N, nucleus. (Scale bars for A and B = 1 µM, and for C and D = 200 nM.)