Uncoupling of T-cell effector functions by inhibitory killer immunoglobulin-like receptors -- Henel et al. 107 (11): 4449 Data Supplement - Supplemental materials for: Henel et al -- Blood

Uncoupling of T-cell effector functions by inhibitory killer immunoglobulin–like receptors
Blood Henel et al. 107: 4449

Supplemental materials for: Henel et al

Files in this Data Supplement:

Figure S1. The anti-KIR2DL2 antibody CH-L does not block the inhibitory effect of KIR2DL2 (PDF, 20.3 KB) -
KIR2DL2⁺ T cells were coincubated with either SEB-coated 721.221 cells or with 721.221/HLA-Cw3 cells. T cells were incubated for 30 minutes at 4°C with the anti-KIR2DS2/DL2/3 mAb CH-L, washed twice with PBS, and then resuspended in complete media. 50,000 T cells were co-cultured with 25,000 SEB-coated 721.221/HLA-Cw3 or 721.221 cells for 48 hours. IFN- secreted into the supernatant was quantified by ELISA. Results are shown as mean ± SD of triplicate cultures. CH-L at the concentration used did not block KIR2DL2 function and was therefore used with the same experimental conditions in the confocal imaging experiments.