Saez et al. 10.1073/pnas.0702259104.

Supporting Information

Files in this Data Supplement:

SI Figure 6
SI Figure 7
SI Movie 1
SI Figure 8
SI Movie 2
SI Figure 9
SI Figure 10




SI Figure 6

Fig. 6. Histogram of the angular distribution of the cellular assemblies on PS substrates. The horizontal direction (0°) corresponds to the long axis of the oval pillars. Twenty percent of the islands were elongated following a 0° ± 15° direction (sector delimited by the dashed line).





SI Figure 7

Fig. 7. Orientation of cell divisions on PDMS anisotropic substrates. (A) Immunofluorescent image of tubulin. The arrows indicate two different events of cell division. (Scale bar: 20 mm.) (B) Histogram of the angular distribution of cell divisions with respect to the direction of highest rigidity (0° = horizontal). Thirteen percent of the islands were elongated following a 0° ± 15° direction (sector delimited by the dashed line).





SI Figure 8

Fig. 8. Dynamics of epithelial growth on anisotropic substrates. Cellular growth in the direction of highest rigidity appears to be driven by the action of leading cells at both lateral edges of an assembly. Time interval between to consecutive images is 800 min. (Scale bar: 20 mm.)





SI Figure 9

Fig. 9. Distribution of cell movements after hepatocyte growth factor treatment. Histogram of the angular distribution of cell movements with respect to the horizontal direction on micropatterned fibronectin substrates. Sixteen percent of the displacement vectors (computed between two consecutive images) were oriented along the 0° ± 15° direction (sector delimited by the dashed line).





SI Figure 10

Fig. 10. Comparison of the responsiveness to substrate anisotropic rigidity of assembly orientation (A) and cell migration (B). The angular distributions shown in Figs. 1C and 5B were fitted (A and B, respectively, black line) with the function y = [C.k(q)]a, where k(q) is the stiffness along direction q, and C and a are the fitting parameters. The values of C, a, and R2 are shown. Cell-assembly orientation appears to be governed by substrate anisotropy in a much more significant way than is the orientation of cell migration (a = 4.2 and 0.72, respectively).





SI Movie 1

Movie 1. Epithelial growth on anisotropic substrates. MDCK cells were plated on fibronectin-coated pillars having an oval cross-section (major axis oriented horizontally) and growth was recorded over a 46-h period. (Scale: 4 mm between two pillars.)





SI Movie 2

Movie 2. Hepatocyte growth factor-induced cell migration on anisotropic substrates. Time-lapse sequence showing the dissociation of an initial island of ~30 MDCK cells cultured on pillars having an oval cross-section (major axis oriented horizontally) after hepatocyte growth factor treatment. Duration: 20 h. (Scale: vertical width of the initial island = 100 mm.)