NF-{kappa}B2 mutation targets TRAF1 to induce lymphomagenesis -- Zhang et al. 110 (2): 743 Data Supplement -

Supplemental materials for Zhang et al, Vol. 110, Issue 2, 743-751 -- Blood

Blood, Vol. 110, Issue 2, 743-751, July 15, 2007

NF- ${kappa}$ B2 mutation targets TRAF1 to induce lymphomagenesis
Blood Zhang et al. 110: 743

Supplemental materials for Zhang et al, Vol. 110, Issue 2, 743-751

Files in this Data Supplement:

Table S1. Lymphoma development in p80HT transgenic founder mice (PDF, 9.61 KB)
Figure S1. Identification of p80HT transgenic founder mice by Southern blot (top) and PCR (bottom) analyses of tail DNA (JPG, 43.6 KB) -
The position of the bands derived from the p80HT transgene is indicated. Copy numbers were determined using the human p80HT cDNA.
Figure S2. p80HT mice showed normal ratios of B cell populations in the bone marrow (JPG, 55.5 KB) -
(A) Flow cytometry analysis of B-cell populations in the bone marrow from a pair of 6-week-old wild-type (WT) and p80HT mice. The percentages of pre- and pro-B cells (B220^lowIgM^−), immature B cells (B220^lowIgM⁺), and mature B cells (B220^highIgM⁺) are indicated. (B) Data from 5 pairs of wild-type (WT) and p80HT mice are summarized. Bars represent means (± SD).
Figure S3. p80HT splenic B cells showed normal proliferative response to LPS (JPG, 31.3 KB) -
Splenic B cells from 6-week-old wild-type (WT) and p80HT mice were cultured for 48 hours at 105 cells/well (96-well plate) in the presence of different concentration of LPS (0.02-20 µg/mL) and pulsed for 12 hours with ³H-thymidine (1 µCi/well). Cells were then harvested for determination of ³H-thymidine incorporation. Data represent the means (± SD) from 4 pairs of mice.