A Novel AAK1 Splice Variant Functions at Multiple Steps of the Endocytic Pathway -- Henderson and Conner 18 (7): 2698 Data Supplement - Supplemental Material -- Molecular Biology of the Cell

Supplemental Material

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Supplemental Figure 1 - AP-2 localization is unaffected by AAK1L CBD2 overexpression. tTA HeLa cells were infected with control tTA (A), full-length AAK1s (B), AAKL (C), or AAK1L CBD2 (D) encoded adenoviruses. Following a 24 hr infection, cells were processed for immunolocalization AP-2 as described for clathrin (Figure 3). AP-2 was detected with the monoclonal antibody AP.6. Non-overexpressing cells are indicated with an asterisk. scale bar, 20 µm