Total P. syringae pv. tomato DC3000 (DC3000) genomic DNA was nebulized and size-fractionated on agarose gels, and after polishing of the ends with Bal 31 exonuclease and T4 DNA ligase, the DNA was ligated to modified pUC vector as described previously (1). Two libraries, a small insert library (2–3 kbp) and a large insert library (6–14 kbp), were constructed and end sequences were generated using ABI BigDye terminator chemistry using ABI 3700 sequencers (Applied Biosystems). The sequences (73,744 random sequences; average edited length of 697 nt) were assembled by using the TIGR assembler program (2). Sequencing and physical gaps were closed by using a combination of primer walking, transposon-mediated sequencing, PCR, and sequencing of PCR products.