Fig. 7.
(AC) Immunofluorescence showing the localization of the nod-khc-LacZ reporter (ref. 1) (red) and DNA (green) in early egg chambers of WT (A), fs(2)TW11 (B), and g Tub23CPI (C) females. Posterior is to the right. The reporter is localized at the posterior side of the egg chamber in all three genotypes (arrows). Not all the germ cells are visible in these confocal sections. (D and E) Ovary from an adult g Tub23CPI fs(2)TW11 female (D) and apical portion of a WT ovary (E) stained with DAPI. Only empty terminal filaments and piles of somatic cells (bracket) can be observed in the double mutant ovaries. (Scale bar, 50 m m.)1. Clark, I. E., Jan, L. Y. & Jan, Y. N. (1990) Development (Cambridge, U.K.) 124, 461470.