Fig. 6.
KSHV and EBV status of B cell tumor samples determined by using PCR. Only samples derived from PEL tested positive for KSHV. The cellular GAPDH gene can be detected in all samples. The size markers vary in length by increments of 100 bp from the 500-bp band (marked). Sequences were amplified from 100 ng of DNA by using the following primers:Gene | 5' primer | 3' primer |
KSHV ORF 33 | GTTTCTGGAGGAAGCATCCG | CGACTGCGCAGAACAGTACC |
EBV gp350 | TGTCAGTACACCATCCAGAG | GACATCTACAGAATTCCAGGC |
GAPDH | ATGGGGAAGGTGAAGGTCGG | TGGTGAAGACGCCAGTGGAC |