Attema et al. 10.1073/pnas.0704468104.

Supporting Information

Files in this Data Supplement:

SI Figure 5
SI Figure 6
SI Figure 7
SI Figure 8
SI Figure 9




SI Figure 5

Fig. 5. Representative FACS profiles of HSC, MPP, CLP, CMP, GMP, MEP, and EP subpopulations of cells purified from adult mouse BM using FACS. (A, C, and D) c-Kit enriched BM. (B) Lineage-depleted or CD27-enriched. (A, B, and C) Staining with purified anti-CD3, -CD4, -CD5, -CD8, -B220, -Gr1, -Mac1, and -TER119 lineage mixture antibodies. (C) Purified anti-IL7Ra antibody was included in the lineage mixture for CMP, GMP, and MEP isolation. (D) Purified anti-TER119 antibody was excluded from the lineage mixture for EP isolation.





SI Figure 6

Fig. 6. Enrichment of active chromatin at promoters of bactin and albumin genes. ChIP assays using antibodies specific for H3K4me2, H3K4me3, and AcH3 of 50,000 hematopoietic stem and progenitor cell populations. The region-specific enrichment at b-actin (stippled box) and albumin (black box) promoters is shown. Background immunoprecipitation (value obtained with IgG) was subtracted from the signals obtained from antibodies to the modified histones. Error bars represent the mean ± SD of three independent ChIP experiments in triplicate qPCR reactions.





SI Figure 7

Fig. 7. The albumin enhancer is methylated in early hematopoiesis but not in liver cells. The DNA methylation status of the albumin enhancer was assessed using BS analysis in HSC, MPP, CLP, CMP, GMP, MEP, and liver cells. The percent methylation of each CpG dinucleotide was determined by the number of methylated clones in the total number of clones sequenced as indicated in the heatmap ranging from 100% methylated (red) to 0% methylated (green).





SI Figure 8

Fig. 8. DNA methylation densities of the Ptrca locus and surrounding CpG islands. The percent methylation of each CpG dinucleotide was determined by the number of methylated clones in the total number of clones sequenced as indicated in the heatmap ranging from 100% methylated (red) to 0% methylated (green).





SI Figure 9

Fig. 9. The Gata3 promoter is located within a CpG island. The DNA methylation status of the Gata3 promoter was assessed using BS analysis in HSC, MPP, CLP, CMP, GMP, MEP, and liver cells. The percent methylation of each CpG dinucleotide was determined by the number of methylated clones in the total number of clones sequenced as indicated in the heatmap ranging from 100% methylated (red) to 0% methylated (green).