Supporting Information

Files in this Data Supplement:

Fig. 7. Forskolin and db-cAMP induce differentiation in C6 glioma cells. (A) C6 cells were incubated with 1.5% FBS-DMEM (a), 10 mM forskolin (b), or 1 mM db-cAMP (c) for 48 h for morphology (original magnification, ´200) . (B) MTT absorbance analyses. (C, Western blotting analyses. (D) Cell-cycle analyses.

Fig. 8. siCREB relieves cell proliferation inhibition induced by cholera toxin. C6 cells were pretreated with siCREB for 48 h and then treated with 10 ng/ml cholera toxin (CT) for an additional 48 h for MTT absorbance. Results are means ±SD (n = 9). Statistical differences are given as *P < 0.01 vs. control and ^#P < 0.01 vs. cholera toxin group.

Fig. 9. Cholera toxin B subunit dose not induce differentiation in C6 glioma cells. (A) C6 cells were incubated with 1.5% FBS-DMEM (a), 10 ng/ml (b), or 10 mg/ml (c) cholera toxin B (CTB) subunit for 48 h for morphology (original magnification, ´200). (B) MTT absorbance analyses. (C) Western blotting analyses.