Microtubule Motor Ncd Induces Sliding of Microtubules In Vivo -- Oladipo et al. 18 (9): 3601 Data Supplement - Supplemental Materials -- Molecular Biology of the Cell

Supplemental Materials

This article contains the following supporting material:

Supplemental Figure 1 - Overexpression of GFP-tagged N-terminal tail domain or C-terminal motor domain does not affect organization of microtubules. Left, MT distribution visualized by immunostaining with α-tubulin antibody; Right, GFP fluorescence. Unlike overexpression of Ncd rigor mutant, neither Ncd N-terminal tail domain (top pair of images) nor C-terminal motor domain (middle pair of images) causes the formation of MT bundles. The expected microtubule binding of GFP-tagged Ncd tail and motor domains seems to be obscured due to a high level of background fluorescence.
Supplemental Movie 1 - Lack of movement of taxol-stabilized MTs in a control non-transfected cell.
Supplemental Movie 2 - "Looping" of taxol-stabilized MTs in a cell overexpressing GFP-Ncd".
Supplemental Movie 3 - The movement of fluorescent speckles in taxol-stabilized MTs in a cell overexpressing GFP-Ncd.
Supplemental Movie 4 - The movement of marks on taxol-stabilized MTs generated by photobleaching in a cell overexpressing GFP-Ncd. This movie corresponds to still images shown in Figure 4 (middle column).
Supplemental Movie 5 - Lack of movement of taxol-stabilized MTs in a cell overexpressing GFP-tau.
Supplemental Movie 6 - Lack of movement of taxol-stabilized MTs in a cell overexpressing GFP-kinesin 1.
Supplemental Movie 7 - Lack of movement of taxol-stabilized MTs in a cell overexpressing Ncd rigor mutant.
Supplemental Table 1 - Half-times of fluorescence recovery in the bleached zones.