Table 1. Chemical shift (Cα and Cβ) assignments for free [13C,15N]-NT(9-13), [13C,15N]-NT(8-13) in detergent buffer, [13C,15N]-NT(8-13) in complex with detergent solubilized receptor (sample NT/NTS-1A), and [13C,15N]-NT(8-13) in complex with lipid-reconstituted NTS-1 (sample NT/NTS-1B)
|
Arg8 |
Arg9 |
Pro10 |
Tyr11 |
Ile12 |
Leu13 |
|
Cα |
NT(9–13) NT(8–13) NT/NTS–1A NT/NTS–1B |
— 58.1 ± 0.5 58.6 ± 1.0 60.1 ± 1.0 |
55.1 ± 0.5 56.1 ± 1.0 56.6 ± 1.0 56.1 ± 1.0 |
66.1 ± 1.0 64.1 ± 1.0 64.1 ± 1.0 62.6 ± 1.0 |
60.1 ± 1.0 60.1 ± 0.5 58.1 ± 1.0 58.6 ± 1.0 |
62.6 ± 1.0 61.6 ± 1.0 61.1 ± 1.0 55.6 ± 1.0 |
56.1 ± 1.0 58.1 ± 1.0 57.1 ± 1.0 56.6 ± 1.0 |
Cβ |
NT(9–13) NT(8–13) NT/NTS–1A NT/NTS–1B |
— 33.6 ± 0.5 32.6 ± 1.5 34.1 ± 1.5 |
31.1 ± 0.5 32.1 ± 0.5 32.6 ± 1.0 32.1 ± 1.0 |
33.1 ± 0.5 34.1 ± 1.5 34.1 ± 1.5 36.6 ± 1.5 |
41.6 ± 1.0 41.1 ± 0.5 41.1 ± 1.0 43.6 ± 1.0 |
40.1 ± 0.5 40.1 ± 0.5 41.1 ± 1.5 40.6 ± 1.5 |
43.6 ± 1.0 44.1 ± 0.5 43.1 ± 1.0 46.1 ± 1.0 |
Δδ |
NT(9–13) NT(8–13) NT/NTS–1A NT/NTS–1B |
— –1.8 ± 1.0 –0.3 ± 2.5 –0.3 ± 2.5 |
–2.3 ± 1.0 –2.3 ± 1.5 –2.3 ± 2.0 –2.3 ± 2.0 |
1.5 ± 1.5 –1.5 ± 2.5 –1.5 ± 2.5 –5.5 ± 2.5 |
–0.4 ± 2.0 0.1 ± 1.0 –1.9 ± 2.0 –3.9 ± 2.0 |
–0.5 ± 1.5 –1.5 ± 1.5 –3.0 ± 2.5 –8.0 ± 2.5 |
–0.8 ± 2.0 0.7 ± 1.5 0.7 ± 2.0 –2.8 ± 2.0 |
ψ φ |
–34 ± 15 — |
136 ± 25 –134 ± 31 |
146 ± 15 –147 ± 16 |
131 ± 19 –121 ± 22 |
134 ± 24 –114 ± 8 |
— –104 ± 16 |
All values are given in ppm. Dihedral angles (ψ,φ
) as obtained from an analysis using TALOS are indicated.