Table 1. Chemical shift (Cα and Cβ) assignments for free [13C,15N]-NT(9-13), [13C,15N]-NT(8-13) in detergent buffer, [13C,15N]-NT(8-13) in complex with detergent solubilized receptor (sample NT/NTS-1A), and [13C,15N]-NT(8-13) in complex with lipid-reconstituted NTS-1 (sample NT/NTS-1B)

 

Arg8

Arg9

Pro10

Tyr11

Ile12

Leu13

Cα

NT(9–13)

NT(8–13)

NT/NTS–1A

NT/NTS–1B

58.1 ± 0.5

58.6 ± 1.0

60.1 ± 1.0

55.1 ± 0.5

56.1 ± 1.0

56.6 ± 1.0

56.1 ± 1.0

66.1 ± 1.0

64.1 ± 1.0

64.1 ± 1.0

62.6 ± 1.0

60.1 ± 1.0

60.1 ± 0.5

58.1 ± 1.0

58.6 ± 1.0

62.6 ± 1.0

61.6 ± 1.0

61.1 ± 1.0

55.6 ± 1.0

56.1 ± 1.0

58.1 ± 1.0

57.1 ± 1.0

56.6 ± 1.0

Cβ

NT(9–13)

NT(8–13)

NT/NTS–1A

NT/NTS–1B

33.6 ± 0.5

32.6 ± 1.5

34.1 ± 1.5

31.1 ± 0.5

32.1 ± 0.5

32.6 ± 1.0

32.1 ± 1.0

33.1 ± 0.5

34.1 ± 1.5

34.1 ± 1.5

36.6 ± 1.5

41.6 ± 1.0

41.1 ± 0.5

41.1 ± 1.0

43.6 ± 1.0

40.1 ± 0.5

40.1 ± 0.5

41.1 ± 1.5

40.6 ± 1.5

43.6 ± 1.0

44.1 ± 0.5

43.1 ± 1.0

46.1 ± 1.0

Δδ

NT(9–13)

NT(8–13)

NT/NTS–1A

NT/NTS–1B

–1.8 ± 1.0

–0.3 ± 2.5

–0.3 ± 2.5

–2.3 ± 1.0

–2.3 ± 1.5

–2.3 ± 2.0

–2.3 ± 2.0

1.5 ± 1.5

–1.5 ± 2.5

–1.5 ± 2.5

–5.5 ± 2.5

–0.4 ± 2.0

0.1 ± 1.0

–1.9 ± 2.0

–3.9 ± 2.0

–0.5 ± 1.5

–1.5 ± 1.5

–3.0 ± 2.5

–8.0 ± 2.5

–0.8 ± 2.0

0.7 ± 1.5

0.7 ± 2.0

–2.8 ± 2.0

ψ

φ

–34 ± 15

136 ± 25

–134 ± 31

146 ± 15

–147 ± 16

131 ± 19

–121 ± 22

134 ± 24

–114 ± 8

–104 ± 16

All values are given in ppm. Dihedral angles (ψ,φ) as obtained from an analysis using TALOS are indicated.