Fig. 6. The ability of IFNAR1- ^/- dendritic cells (DCs) to stimulate antigen-specific proliferation of naive CD8⁺ T cells. Lipopolysaccharide (LPS)- or poly(I:C)-stimulated WT or IFNAR1- ^/- DCs were irradiated (30 Gy) and plated at 10⁴ cells per well with MACS-purified CD8⁺ T cells (5 ´ 10⁴) from the lymph nodes of 2C-transgenic mice. The synthesized peptide SIYRYYGL, which is recognized by CD8⁺ T cells from 2C-tg mice, was added at the indicated concentration. Data are expressed as mean ± SD of [³H]thymidine uptake.