>Sequence of 21B3, the P450 variant used to start the equilibration evolution.  This P450 has been evolved from the wildtype P450 BM3 heme domain to efficiently hydroxylate 12-p-nitrophenoxydodecanoic acid, as described in: P. C. Cirino and F. H. Arnold, "A self-sufficient peroxide-driven hydroxylation biocatalyst." Angew. Chem. Int. Ed. 42:3299-3301 (2003).  This P450 was used as the initial parent to start the equilibration evolution experiments.
ATGACAATTAAAGAAATGCCTCAGCCAAAAACGTTTGGAGAGCTTAAAAATTTACCGTTATTAAACACAGATAAACCGGTTCAAGCTTTGATGAAAATTGCGGATGAATTAGGAGAAATCTTTAAATTCGAGGCGCCTGGTCGTGTAACGCGCTACTTATCAAGTCAGCGTCTAgTTAAAGAAGCATGCGATGAATCtCGCTTTGATAAAAACTTAAGTCAAGCGCTTAAATTTGTACGTGATTTTGCAGGAGACGGGTTggccACAAGCTGGACGCATGAAAAAAATTGGAAAAAAGCGCgTAATATCTTACTTCCAAGCTTaAGTCAGCAGGCAATGAAAGGCTATCATGCGATGATGGTCGATATCGCCGTGCAGCTTGTTCAAAAGTGGGAGCGTCTAAATtCAGATGAGCATATTGAAGTACCGGAAGACgTGACACGTTTAACGCTTGATACGATTGGTCTTTGCGGCTTTAACTATCGCTTTAACAGCTTTTACCGAGATCAGCCTCATCCATTTATTACAAGTATGGTCCGTGCACTGGATGAAGCAATGAACAAGCTGCAGCGAGCAAATCCAGACGACCCAGCTTATGATGAAAACAAGCGCCAGTTTCAAGAAGATATCAAGGTGATGAACGACCTAGTAGATAAAATTATTGCAGATCGCAAAGCAAGCGGTGAgCAAAGCGATGATTTATTAACGCACATGCTAcACGGAAAAGATCCAGAAACGGGTGAGCCGCTTGATGACGAGAACATTCGCTATCAAATTATTACATTCTTAATTGCGGGACACGAAACAACAAGcGGTCTTTTAaCATTTGCGCTGTATTTCTTAGTGAAAAATCCACATGTATTACAAAAAGCAGCAGAAGAAGCAGCACGAGTTCTAGTAGATCCTGTTCCAAGCTACAAACAAGTCAAACAGCTTAAATATGTCGGCATGGTCTTAAACGAAGCGCTGCGCTTATGGCCAACTGCTCCTGCGTTTTCCCTATATGCAAAAGAAGATACGGTGCTTGGAGGAGAATATCCTTTAGAAAAAGGCGACGAACTAATGGTTCTGATTCCTCAGCTTCACCGTGATAAAACAATTTGGGGAGACGATGTGGAAGAGTTCCGTCCAGAGCGTTTTGAAAATCCAAGTGCGATTCCGCAGCATGCGTTTAAACCGTTTGGAAACGGaCAGCGTGCGTGTATCGGTCAGCAGTTCGCTCTTCATGAAGCAACGCTGGTACTTGGTATGATGCTAAAACACTTTGACTTTGAAGATCATACAAACTACGAGCTGGATATTgAAGAAACTTTAACGTTAAAACCTGAAGGCTTTGTGaTAAAAGCAAAATCGAAAAAAATTCCGCTTGGCGGTATTCCTTCACCTAGCACTCATCATCATCATCATCATTAA