Regulation of the Formin for3p by cdc42p and bud6p -- Martin et al. 18 (10): 4155 Data Supplement - Supplemental Material -- Molecular Biology of the Cell

Regulation of the Formin for3p by cdc42p and bud6p
Mol. Biol. Cell Martin et al. 18: 4155

Supplemental Material

This article contains the following supporting material:

Supplementary Figure 1
Supplementary Table
Movie 1 - Movement of for3p-3GFP. Widefield images of cells expressing endogenous for3p-3GFP were taken approximately every 550ms. The movie is presented 5x real time. The white bar tracks the movement of one for3p dot.
Movie 2 - Movement of for3pDAD*-2GFP. Widefield images of cells expressing endogenous for3pDAD*-2GFP under the endogenous promoter were taken approximately every 550ms. The movie is presented 5x real time. Note that for3pDAD*-2GFP dots move away from cell tips along linear paths, similar to wildtype for3p. White bars track the movement of a few for3p dots.
Movie 3 - Actin cable dynamics in wildtype cells. Projection images of 3 spinning disk confocal sections taken at 0.5µm interval in the upper half of cells expressing GFP- CHD were taken approximately every 3.5s. The movie is presented 33x real time.
Movie 4 - Actin cable dynamics in for3•FH3 cells. Projection images of 3 spinning disk confocal sections taken at 0.5µm interval in the upper half of for3•FH3-myc cells expressing GFP- CHD_rng2 were taken approximately every 3.5s. The movie is presented 33x real time. Note how the actin cables appear disorganized.
Movie 5 - Movement of myo52p-GFP in for3•FH3 cells. Widefield images of cells expressing endogenous myo52p-GFP were taken approximately every 500ms. The movie is presented 5x real time. Note that myo52p-GFP fails to accumulate at cell tips, but still moves in linear paths in the cell (arrows).