Fig. 6. Analysis of insulin mRNA and protein levels. (A) Quantitative RT-PCR analysis of insulin transcript levels in WT and PASK^-/- islets from 16-week-old male mice. Data represent the mean ± SD of six mice per genotype. RPL13A was used as normalizer. (B) Measurement of total insulin extracted from whole pancreas of 16-week-old male mice. Data represent the mean ± SD of four mice per genotype.

Fig. 7. PASK^-/- mice on HFD exhibited reduced circulating insulin levels and adiposity. (A) Measurement of plasma insulin levels from 24-week-old HFD WT and PASK^-/- male mice fasted for 8 h. Data represent the mean ± SD of 10 mice per genotype. (B) Body fat mass in 23-week-old WT and PASK^-/- mice on HFD, measured by dual energy x-ray absorptiometry. Data represent the mean ± SD of six mice per genotype.