Nakamoto et al. 10.1073/pnas.0707484104.

Supporting Figures

Files in this Data Supplement:

SI Figure 5
SI Figure 6
SI Figure 7




SI Figure 5

Fig. 5. No statistically significant effect was detected by adding translation inhibitors or transcription inhibitor on GluR1 internalization in untreated wild-type dendrites without DHPG treatment. The duration of incubation and concentrations of the drugs were the same as used in Fig. 1 (also see Materials and Methods). Columns indicate averages of IF signals in dendrites (n = 21 per column), and error bars indicate standard deviations. Terms in the legend and P values compared to wild-type (= no treatment) dendrites examined with two-tailed ANOVA (a = 0.05) are as follows: WT, wild-type; aniso, anisomycin, P = 0.169; cyclo, cycloheximide, P = 0.105; puro, puromycin, P = 0.065; action, actinomycin D, P = 0.287.





SI Figure 6

Fig. 6. Quantification of GluR2 IF signals. Mean IF signals of internalized (A), surface-remaining (B), and i/t GluR2 (C) in si-luc- (open columns) or si-fmr1- (solid columns) transfected dendrites are graphed. Columns indicate averages of IF signals in dendrites (n = 15 per column), and error bars represent standard deviations. Two-tailed ANOVA (a = 0.05) P values: (A) *, 2.2 ´ 10-2; (B) *, 4.8 ´ 10-4; (C) *, 2.6 ´ 10-4.





SI Figure 7

Fig. 7. Quantification of GluR1 IF signals in dendrites transfected with si-luc or si-fmr1 without or with MPEP treatment. Columns indicate averages of IF signals in dendrites (n = 21 per column), and error bars indicate standard deviations. Two-tailed ANOVA (a = 0.05) P values: (A) *, 1.6 ´ 10-3; **, 4.4 ´ 10-7. (B) *, 1.9 ´ 10-4; **, 3.6 ´ 10-7. (C) *, 2.0 ´ 10-11; **, 3.4 ´ 10-16. (D) No statistically significant difference was detected in total labeled GluR1.