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Video 4
Thin peripheral region of a PC12 cell. A thin peripheral region of a PC12 cell, transfected with GFP-peripherin for 24 h and treated with NGF for 2 h was monitored every 3 s for a period of 21 s. Images are displayed at 30 frames/s. Immediately after the last frame was captured, this cell was fixed on the microscope stage with formaldehyde and prepared for FISH. FISH image is shown in Fig. 2 G.