Figure S1. CpG-induced cell adhesion requires actin rearrangement. THP-1 cells were plated at 1 x 105 cells/well in 96-well microtiter plates. Cells were pretreated for 10 min with increasing concentrations of cytochalasin D, jasplakinolide, and latrunculin A and stimulated with 1 µg/ml CpG for 20 min. After fixation, cells were washed six times and nuclei were stained with TOPRO-3. Adhesion was then quantified as described in Materials and methods.