Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis -- Data Supplement - JCB--Yang et al. -- The Journal of Cell Biology

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Figure S2. Distribution of STAG3 and γH2AX in wild-type and Sycp2^-/- spermatocytes. Surface-spread nuclei of spermatocytes were immunostained with anti-STAG3 serum (green) or γH2AX-specific antibodies (green), CREST (red), and DNA (blue). We found no difference in the amount of γH2AX between wild-type and Sycp2^-/- spermatocytes, suggesting that absence of axial elements in Sycp2^-/- spermatocytes does not abolish the formation of DSBs. (A) STAG3 localization in wild type. (C) STAG3 localization in Sycp2^-/- spermatocytes. (E) γH2AX staining in wild type. (G) γH2AX staining in Sycp2^-/- spermatocytes. (B, D, F, and H) DAPI staining of the same cells shown in A, C, E, and G, respectively.