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Video 1
Time-lapse widefield epifluorescence microscopy of MDA-MB-231 American Type Culture Collection breast adenocarcinoma cells. Bidirectional motility of GFP-Rab21-positive vesicles moving between the plasma membrane and cell periphery. Colored lines are tracking three individual vesicles moving to the enlarged endosome (green), to the cell periphery (blue), and toward the leading edge (red). Frames were collected at 0.6-s intervals and are displayed at 12 frames/s. Still images can be found in Fig 4 A.